FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001858573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001858573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1552023
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC232561.4984314021119531No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT147080.9476663683463454No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA125760.8102972700791161No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT118130.7611356275003657No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC115010.7410328326319907No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA110420.7114585286429389No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT104600.6739590843692393No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT104410.6727348757073832No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC100780.6493460470624468No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA97310.6269881309748631No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT95680.6164857092968339No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC90490.58304548321771No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG88770.57196317322617No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG85330.5497985532430898No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC73960.47653932963622314No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA70840.4564365347678481No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA70460.4539881174441358No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC70210.45237731657327246No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA68940.44419444814928644No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC68300.44007079791987624No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC65970.4250581338034295No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC64100.41300934328937133No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC63400.4084991008509539No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT62130.4003162324269679No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG59700.3846592479621758No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC58360.3760253552943481No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG57600.3711285206469234No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT57270.36900226349738374No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA54470.3509612937437138No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG54330.3500592452560304No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC52640.3391702313689939No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA52170.3361419257317707No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG51510.3318894114326914No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG49310.3177143637690936No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT49150.316683451211741No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG48690.31371957760935243No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT46750.3012197628514526No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT46530.2998022580850928No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC45510.2932301905319702No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT45140.2908462052430924No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT45050.2902663169295816No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT44990.2898797247205743No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA43770.28201901647076105No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG43320.279119574903207No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGG42100.27125886665339366No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC40830.2630759982294077No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC40170.25882348393032834No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC39830.25663279474595413No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGA39020.25141379992435675No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG38910.25070504754117695No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA38900.25064061550634237No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT38030.24503502847573777No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTT37840.2438108198138816No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT36510.23524135918088843No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG35940.23156873319531993No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG35060.22589871412988083No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGA35000.22551212192087358No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT34890.2248033695376937No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC33630.21668493314854226No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC33200.21391435565065722No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG32280.20798660844588No Hit
GGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTT30880.19896612356904506No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT30850.19877282746454142No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC30710.19787077897685795No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA30690.19774191490718887No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT30150.19426258502612395No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG29960.1930383763642678No Hit
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCT28990.18678846898531787No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG28470.18343800317392203No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA28140.18131174602438238No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC28070.1808607217805406No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG27730.1786700325961664No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG27500.1771880957949721No Hit
TTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCT27210.1753195667847706No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGT27030.17415979015774893No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG26730.1722268291127129No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCC26490.17068046027668404No Hit
GTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGG26480.17061602824184952No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT26450.1704227321373459No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCG26410.17016500399800777No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC26220.1689407953361516No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT25630.165139305280914No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG25390.16359293644488518No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA24980.16095122301666923No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGC24910.16050019877282745No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA24780.15966258231997849No Hit
ACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT24750.1594692862154749No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC24730.15934042214580585No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT24260.15631211650858268No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTG24230.15611882040407907No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGT24080.15515233988156102No Hit
CCGCTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGAT23640.15231733034884148No Hit
ATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCG22640.14587412686538795No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA22410.14439219006419363No Hit
CACACATACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC22390.14426332599452457No Hit
AAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAG22320.14381230175068282No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT22090.1423303649494885No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA22080.14226593291465395No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCC22070.14220150087981945No Hit
GAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG22010.14181490867081223No Hit
AAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCT21380.13775569047623648No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC21200.13659591384921488No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG21070.1357582973963659No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA21060.13569386536153136No Hit
GGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGAT21020.13543613722219322No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACG21010.1353717051873587No Hit
GGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTGATTTC20680.13324544803781901No Hit
TTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTC20660.13311658396814996No Hit
GTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA20490.13202123937596286No Hit
GTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACACGGTGGAT20360.1311836229231139No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC20050.1291862298432433No Hit
CTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACC19800.12757542897237992No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG19560.12602906013635107No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACG19420.12512701164866757No Hit
CCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGT18860.1215188176979336No Hit
GCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTGATTTCTTTTC18840.12138995362826453No Hit
GTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAA18750.1208100653147537No Hit
GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAC18530.11939256054839395No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT18310.11797505578203415No Hit
CCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATT18200.11726630339885427No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATA18110.11668641508534344No Hit
GGGGAAACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGT17980.11584879863249448No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA17440.11236946875142959No Hit
GTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT17400.11211174061209145No Hit
ACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA17390.11204730857725691No Hit
AATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGAT17230.11101639601990435No Hit
GTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC17150.11050093974122807No Hit
ACCCTGTATCGCACGCCTTTCCAGACGCTTCCACTAACACACACACTGAT17120.11030764363672446No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGG16900.10889013887036467No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGA16520.10644172154665234No Hit
GTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC16450.1059906973028106No Hit
TTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGG16450.1059906973028106No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATG16440.10592626526797605No Hit
GAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCC16390.10560410509380337No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA16360.10541080898929978No Hit
CCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAG16230.10457319253645081No Hit
GATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT16210.10444432846678174No Hit
CTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCG16190.10431546439711267No Hit
ACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCT15980.10296239166558743No Hit
GCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACG15850.10212477521273848No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)