FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001860188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001860188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566946
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA254100.7123741149992178Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT225940.6334270269300404Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT121830.34155268961178553Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG106850.29955597869998596Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG87080.24413041296391927Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC77570.21746894962805718Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC73860.20706789505644327Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG68480.19198496416822683Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA54860.15380103875976817No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG50330.14110109881113983Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT50070.14037218393550113Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG47510.13319517592921226No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG43640.12234555835720529No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT43410.12170074904414027No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40460.11343036872439335Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA38740.10860831647016803No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC37610.10544034027989209No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA36890.10342180677812335Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT36740.10300127896525488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)