FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001860316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001860316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206216
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63910.289681518038125No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41210.18679041399391536Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40310.182711031014189No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC36340.16471641942584045Clontech Universal Primer Mix Long (96% over 25bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA31870.14445548395986613No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC29490.13366778230236748No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC27800.12600760759599242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25290.11463066173031108No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA24740.11213770546492274No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA24480.11095921704855735Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC22490.10193924801560682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)