FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001860990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001860990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871928
Sequences flagged as poor quality0
Sequence length151
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82240.9431971447183712Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT67240.7711645915717812Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT41270.4733188978906515Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG35800.41058436017652833Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA29060.3332843996293272Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG27640.3169986512647833Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG25000.2867209219109835Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24300.27869273609747597Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC23230.26642108063968584Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC22600.25919571340752906Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT17350.19898431980622253Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG13530.15517336293822426Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC13180.15115927003147048Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG12050.13819948436109403Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA11810.13544696351074859Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11290.12948316833500012Clontech Universal Primer Mix Long (96% over 25bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG10590.1214549825214926No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10370.11893183840867595No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10220.11721151287721004Clontech Universal Primer Mix Long (96% over 26bp)
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10190.11686744777091686No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG9870.11319741997045628Clontech Universal Primer Mix Long (96% over 26bp)
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC9810.11250928975786992No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC9460.10849519685111615Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC9190.10539861089447754Clontech Universal Primer Mix Long (96% over 26bp)
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG9190.10539861089447754No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9120.10459579231312677No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT8970.10287546678166086Clontech Universal Primer Mix Long (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC8970.10287546678166086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA8900.1020726482003101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)