FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001861195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001861195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034665
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT145781.4089584551521508No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC118291.1432685941826581No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT79520.7685579390430719No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC79260.7660450483973074No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT78580.7594728728622308No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA74830.7232292577790879No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC73550.7108581038307085No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT67420.6516118743747977No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC66660.6442665017179473No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC65930.6372110779817622No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC64330.6217471355462879No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA64010.6186543470591931No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT60290.5827006808967153No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG58660.5669467895405759No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT58490.5653037456568067No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA57160.5524493435073188No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA57140.5522560442268754No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG55420.5356323061087405No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT55400.5354390068282971No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC51800.50064513634848No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC50640.4894337780827611No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA45670.44139890689256905No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC44250.4276746579810857No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC43470.420135986043792No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG41370.39983956159723194No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGA40910.3953936781470331No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT40120.3877583565695177No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA39220.3790598889495634No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA38990.3768369472244639No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG36740.35509077817457824No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC36540.353157785370144No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA35760.34561911343285023No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG35220.34040003286087767No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC35100.3392402371782171No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA34710.33547090120957024No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT34700.3353742515693485No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG33540.3241628933036297No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG31900.30831235230726856No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC31120.3007736803699748No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT30870.29835743936443193No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC30570.29545795015778054No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG29970.2896589717444777No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT29910.2890790739031474No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC28510.2755481242721074No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT28380.2742916789492251No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG28120.27177878830346053No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC27800.2686859998163657No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA27710.26781615305437023No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC27610.26684965665215316No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG27560.26636640845104453No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA25980.2510957652960137No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT25600.24742307896758856No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGT24840.24007770631073824No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT24830.23998105667051656No Hit
CACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTG24280.23466532645832228No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG24280.23466532645832228No Hit
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCT23880.23079934084945367No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA22420.2166884933770834No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG21910.21175936172577597No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG21830.21098616460400224No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC21790.21059956604311542No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCC21450.2073134782755771No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT21370.20654028115380338No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA21260.20547713511136456No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC20650.19958150705783997No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT20090.19416912720542398No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGG20070.19397582792498055No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGA19910.19242943368143312No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC19840.19175288619988112No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT19550.1889500466334514No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA19530.188756747353008No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT19420.18769360131056914No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA19280.18634050634746513No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG18880.18247452073859655No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT18580.17957503153194512No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT18560.1793817322515017No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG18520.17899513369061482No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA18460.17841523584928454No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG18460.17841523584928454No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC18000.17396935239908567No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA17900.17300285599686854No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGA17760.17164976103376453No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG16970.16401443945624913No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG16740.1617914977311497No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT16350.15802216176250283No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC16340.15792551212228112No Hit
GGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTAC16320.1577322128418377No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGT16210.15666906679939885No Hit
ACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT15940.15405952651341254No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCC15800.15270643155030855No Hit
GGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGA15500.14980694234365713No Hit
GTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATTCAGG15470.14951699342299196No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATA15380.14864714666099654No Hit
CCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGT15360.14845384738055314No Hit
GAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAG15240.14729405169789256No Hit
GAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAA14680.14188167184547656No Hit
GGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTT14590.14101182508348112No Hit
TTTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCT14060.13588939415173026No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTG14040.13569609487128684No Hit
GTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA14020.1355027955908434No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCG13640.13183010926241825No Hit
CTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCG13590.1313468610613097No Hit
AAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAG13240.1279641236535497No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTT13240.1279641236535497No Hit
ATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCG13210.12767417473288456No Hit
AAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCT13130.12690097761111085No Hit
GGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGAT13120.1268043279708891No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACG13120.1268043279708891No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCA12850.12419478768490284No Hit
CTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACC12780.12351824020335085No Hit
CACTAACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGC12760.12332494092290741No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA12550.12129529847825142No Hit
AATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGAT11910.11510972150406171No Hit
GTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACACGGTGGAT11810.11414322510184456No Hit
GTACATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCA11770.1137566265409577No Hit
GGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACAC11770.1137566265409577No Hit
GTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAA11710.1131767286996274No Hit
GCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCG11710.1131767286996274No Hit
CACACATACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC11660.11269348049851885No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA11620.112306881937632No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA11390.11008394021253257No Hit
GTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11290.1091174438103154No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT11280.1090207941700937No Hit
CCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGATT11130.107571049566768No Hit
GATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTT10990.10621795460366398No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC10970.10602465532322056No Hit
GGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTGATTTC10970.10602465532322056No Hit
CAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC10960.10592800568299886No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTG10930.1056380567623337No Hit
CAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGA10870.10505815892100341No Hit
ACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCT10800.10438161143945142No Hit
ACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAG10730.10370506395789943No Hit
ACCCTGTATCGCACGCCTTTCCAGACGCTTCCACTAACACACACACTGAT10660.10302851647634742No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACC10630.10273856755568228No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT10630.10273856755568228No Hit
GTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGT10420.10070892511102626No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTA10410.10061227547080455No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)