FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001862480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001862480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1048558
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT83230.7937567592827482No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA75450.7195596237880976No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC73050.6966710472858917No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT71960.6862758187911399No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT59580.5682089116672612No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC51050.486859096015671No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG50090.4777036654147887No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG47650.45443361263754606No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC46380.44232174090512877No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT44440.4238201415658457No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA39580.37747077414887875No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT36690.3499091132774725No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA36050.3438054928768842No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA32900.313764236217739No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC31160.29717001825363976No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA30840.29411820805334565No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC30600.2918293504031251No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT30230.288300694859035No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC30130.2873470041714431No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG30100.28706089696516546No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT29770.2839137176961122No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC28400.270848155276103No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA27640.26360010605040446No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT27580.26302789163784934No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG27010.25759185471857543No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT25560.24376333974849268No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG25540.2435726016109743No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT24770.2362291833165166No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC24430.2329866349787041No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA23840.22735985992191182No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA23620.2252617404092096No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA23520.2243080497216177No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG23090.22020717976497248No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC22740.21686926235840076No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA22520.21477114284569856No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC22090.21067027288905338No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT22080.21057490382029417No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG21270.20285000925079968No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC20760.19798618674408092No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC19450.18549283873662686No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT19380.18482525525531254No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC19300.18406230270523902No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT19050.1816780759862592No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT18990.18110586157370406No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG17880.17051989494143385No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG17130.1633672147844945No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC17010.16222278595938422No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCC16230.15478399859616732No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT16120.1537349388398162No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC15860.15125534305207722No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG15680.14953869981441179No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG15660.1493479616768934No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA15340.1462961514765993No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA15250.14543782985776657No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC14820.14133695990112136No Hit
ACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT14620.13942957852593754No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT14380.13714072087571694No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC14370.13704535180695776No Hit
AACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCC14240.13580555391308827No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG14220.13561481577556989No Hit
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCT13780.13141857675016547No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG13240.12626864703716914No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA12910.12312146776811583No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG12900.12302609869935664No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT12850.12254925335556069No Hit
GTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTG12660.12073724104913605No Hit
AAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCT12390.1181622761926379No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACG12310.11739932364256435No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG11810.1126308702046048No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGT11780.11234476299832723No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC11760.11215402486080885No Hit
GACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACG11760.11215402486080885No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCAT11620.11081885789818018No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA11560.11024664348562503No Hit
GTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC11530.10996053627934746No Hit
GTATCAACGCAGAGTACATGGGGTTAAGCGACTAAGCGTACACGGTGGAT11420.10891147652299633No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT11410.10881610745423714No Hit
ATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCG11310.10786241676664524No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG11070.10557355911642466No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA11040.10528745191014709No Hit
ACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGT10980.10471523749759193No Hit
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT10940.10433376122255517No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA10830.10328470146620405No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC10740.10242637984737135No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC10690.10194953450357538No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG10530.10042362940342832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)