FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001864181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001864181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974721
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC32380.33219762373027767No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT20310.20836731741698394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17230.1767685317131774No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA14270.1464008675302984No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC13950.143117876807825No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT13620.1397322926252743No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA13540.13891154494465596No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA12440.12762626433615365No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12010.12321474555282998Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT11350.11644357718772859No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10390.10659460502030838Clontech Universal Primer Mix Long (96% over 26bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA10180.10444014235868519No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA10050.10310642737768039No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA9880.10136233855636638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)