FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001865195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001865195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1478408
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC111360.7532426772582399No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT87870.59435555002408No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA70900.47956991574720914No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA64830.43851223748789236No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT62740.4243754092239761No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT61110.4133500359846538No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC59240.4007012949064128No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC55270.37384808523763396No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG54590.3692485430273646No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT53610.3626197910184469No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT52390.3543676711706105No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT52110.3524737420252055Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA49890.33745758951520827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT45480.3076282054750786Clontech Universal Primer Mix Long (96% over 26bp)
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA45120.30519315371670064No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC45000.30438146979724134No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC41270.27915162796738113No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA40930.2768518568622464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG40690.2752284890233278No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG39950.2702231048533287No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA37710.25507167169008826No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC37180.251486734379143No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA35680.2413406853859016No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC35380.2393114755872533No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA34090.23058587345306572No Hit
GCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGT33250.22490408601685058Clontech Universal Primer Mix Long (96% over 26bp)
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC32720.22131914870590524No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC32640.2207780260929324No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG32620.22064274543968915No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG32550.22016926315333793No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA32380.21901937760077056No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATC32310.2185458953144193No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC30560.20670883815563768No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG30430.20582951390955678No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT29960.20265041855834112No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG29700.2008917700661793No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC28970.19595402622280184No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG27880.18858123062104643No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA27710.18743134506847908No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTC27040.18289944318483126No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT27010.18269652220496643No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT26410.17863810260766988No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA26340.1781646203213186No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT26330.178096979994697No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGATGTT26240.1774882170551025No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCC25280.17099474569942805No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG25070.16957429884037423No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT24600.16639520348915862No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA23500.15895476756078158No Hit
TTCCAGACGCTTCCACTAACACACACACTGATTCAGGCTCTGGGCTGCTC23250.15726375939524137No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA22960.1553021899232147Clontech SMART CDS Primer II A (96% over 26bp)
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA22650.15320533979794482No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGA22510.15225837522524227No Hit
TATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGAT22260.15056736705970206No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT21610.14617074582929745No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACAT21320.14420917635727082No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG20890.1413006423125416No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT20240.136904021082137No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC19980.13514537258997517No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACATACTGAT19800.1339278467107862No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC19760.13365728540429977No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG19650.13291324181146205Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19600.132575040178354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT19500.1318986369121379No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGA19470.13169571593227308No Hit
GGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGG19110.12926066417389517No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG19100.12919302384727357Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT18850.1275020156817333Clontech Universal Primer Mix Long (96% over 26bp)
AACACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCT18720.12662269143565238No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG18650.12614920914930114No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18650.12614920914930114Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGG18260.12351123641105838Clontech Universal Primer Mix Long (96% over 26bp)
GGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTT18160.1228348331448423No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGG18070.1222260702052478No Hit
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA17550.11870877322092413Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT17360.11742360701511355Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGG17260.11674720374889747Clontech Universal Primer Mix Long (96% over 26bp)
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG17020.11512383590997885No Hit
CCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCG16930.11451507297038435No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG16930.11451507297038435No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC16920.11444743264376275No Hit
GTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATG16800.11363574872430345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA16680.11282406480484412No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT16580.11214766153862803No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16430.1111330566393039Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC16150.10923912749389886Clontech Universal Primer Mix Long (96% over 25bp)
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGAGGCGAAC16150.10923912749389886No Hit
ACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCT16120.109036206514034No Hit
CAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGTGTG16090.10883328553416918No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG15860.10727755802187218No Hit
CATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATAC15850.10720991769525055Clontech Universal Primer Mix Long (96% over 26bp)
TTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCT15830.10707463704200736No Hit
CCGCTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAGAT15690.10612767246930482No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC15550.10518070789660228No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG15510.10491014659011585No Hit
ATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAA15510.10491014659011585Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA15430.10436902397714298No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA15150.10247509483173793Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACG15050.10179869156552183Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT14840.10037824470646803Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)