FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001866316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001866316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1291454
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGGCCCTCAGGCTGTTCAGTTGCAGAGACA57070.4419050155870825No Hit
GTATTACTGTCAGCAGTATGATAGTTCGGTATTCACTTTCGGCCCTGGGA36290.2810011041817982No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT31440.2434465339067439No Hit
GTATATTACTGTGCGAGATGGAGCAGTGACTGGCACGCGGTTGACAACTG28320.21928771756485324No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27650.2140997666196396Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25740.19931023482059754No Hit
GTATGATAGTTCGGTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCA22020.1705054922591126No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21980.170195763844473No Hit
ATCATACTGCTGACAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGC21790.16872455387493476No Hit
CAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGT21330.1651626771065791No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTG20410.15803892356986776No Hit
ATATACAGCCGTGTCCTCGGCCCTCAGGCTGTTCAGTTGCAGAGACAGTG20410.15803892356986776No Hit
GTCCTACTAGTACTATGAGAAATGTATGAAACCCACTCCAGTCCCTTCCC19490.15091517003315644No Hit
GTACTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGC19240.14897936744165877No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19110.14797275009408No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC18430.14270736704520642No Hit
GTACTAGTAGGACATTGTATGCAGAATCTGTGAAGGGCCGATTCACCATT18230.1411587249720083No Hit
GTAATACACTGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA17770.13759684820365264No Hit
TACTAGTACTATGAGAAATGTATGAAACCCACTCCAGTCCCTTCCCTGGA17610.13635793454509412No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA17560.1359707740267946Clontech SMART CDS Primer II A (96% over 26bp)
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT17540.1358159098194748No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC17480.13535131719751536Clontech Universal Primer Mix Long (96% over 25bp)
ATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGG17240.13349294670967762No Hit
TTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCC16540.1280726994534842No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16360.1266789215876059Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT16150.12505284741074787Clontech Universal Primer Mix Long (96% over 26bp)
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15790.12226529167899128No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC15460.11971003225821439No Hit
ATATTACTGTGCGAGATGGAGCAGTGACTGGCACGCGGTTGACAACTGGG15410.11932287173991485No Hit
CTATCATACTGCTGACAGTAATACACTGCAAAGTCTTCAGGCTCCAGTCT15020.11630301969717853No Hit
TACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCAT14660.1135154639654219No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14650.113438031861762No Hit
CTCCTGTACAGCCTCTGGGTTCACCTTCAGTACTTTTAACATGAACTGGG14520.11243141451418323No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14440.111811957684904No Hit
TCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGC14180.10979872298974645No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA14140.10948899457510682No Hit
GTGTATTACTGTCAGCAGTATGATAGTTCGGTATTCACTTTCGGCCCTGG13810.10693373515432993No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13030.10089403106885728No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)