FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001866461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001866461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214953
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT50690.4172177853793521No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGATAAGGA46780.38503547050791265No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG42910.35318238647914774No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG32350.2662654440130606No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA31560.2597631348702378No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT31540.2595985194489005No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAA27610.22725158915612373No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTC27210.22395928072937799No Hit
GTTCTAAGTTGGTTGTTAATCGCCCGCCGGACGGCCGAAGCCTGCCAAGG24200.19918465981811642No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT23680.19490465886334699No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA21780.17926619383630477No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG21300.1753154237242099No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATC21260.17498619288153533No Hit
CCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCATTTTGCCGACTT21040.1731754232468252No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA20910.1721054230081328No Hit
AGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGATAAGGATTGG20030.16486234446929224No Hit
GTGTAGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGATTC19840.16329849796658802No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGT18590.15301003413300762No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCT18500.15226926473698985No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC18460.15194003389431526No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG17210.14165157006073487No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGA17070.14049926211137387No Hit
ATCCTAAGCTATAGGGAAGTTCCGTTTTAAAGGCGCCCTCGTGCGCCGTG16260.13383233754721374No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG16140.13284464501919005No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT16100.13251541417651547No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCT15720.12938772117110703No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGCCCGCCGGACGG14680.12082771926156814No Hit
TCGCTACACGGCGCACGAGGGCGCCTTTAAAACGGAACTTCCCTATAGCT14480.11918156504819527No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGA14310.11778233396682834No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT14230.11712387228147919No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTA13990.11514848722543178No Hit
CTCTACTTGTGCGCTATCGGTCTCCGGCCAATATTTAGCTTTAGATGAAA13930.11465464096141992No Hit
GTGCTGGGGGGCGCCGGCCGGTTGCCCGGCTAGTGCCCCCCTGCTCCGAG13790.1135023330120589No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGCCAACACCACTT13780.11342002530139025No Hit
TCCTTATCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTATCTACAT13390.11021002458531319No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGCCCGCCGGACGGCCGAAG13110.10790540868659117No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAT12900.10617694676254967No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAAC12690.10444848483850816No Hit
GTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGATAAGGATT12690.10444848483850816No Hit
GGTTAATATTCCGGCACCTGGATGTGGATTCTCCACGGCAACGTAACTGA12630.1039546385744963No Hit
TCCCAACCCCAGTGCTGGGGGGCGCCGGCCGGTTGCCCGGCTAGTGCCCC12590.10362540773182173No Hit
TGCCAAGGGCGTCCCAACCCCAGTGCTGGGGGGCGCCGGCCGGTTGCCCG12260.1009092532797565No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAA12210.10049771472641328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)