FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001866462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001866462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1214953
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT50530.41590086200865384No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGATAAGGA45590.37524085293834414No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG40950.3370500751880937No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA32110.26429005895701313No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTT31230.2570469804181726No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG29560.24330159273650917No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAA27020.22239543422667377No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTC25670.21128389328640695No Hit
GTTCTAAGTTGGTTGTTAATCGCCCGCCGGACGGCCGAAGCCTGCCAAGG23520.19358773549264868No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCA23500.1934231200713114No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACT23090.19004850393389705No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA22400.18436927189776064No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATC21160.1741631157748489No Hit
GTGTAGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGTGGATTC21050.17325773095749383No Hit
GTCCGGTGCGCCCCCGACGATCCTTGAAAATCCGCGGGAAGGAATAGTTT20760.17087080734810317No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTG20560.1692246531347303No Hit
AGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGATAAGGATTGG18960.15605541942774742No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGC18130.14922387944225005No Hit
TAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGT17550.14445003222346872No Hit
CCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCATTTTGCCGACTT17330.1426392625887586No Hit
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAG17330.1426392625887586No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCT17310.14247464716742128No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGA16890.1390177233193383No Hit
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAG16320.1343261838112256No Hit
ATCCTAAGCTATAGGGAAGTTCCGTTTTAAAGGCGCCCTCGTGCGCCGTG15620.12856464406442059No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAATCGCCCGCCGGACGG15200.1251077202163376No Hit
CTCTACTTGTGCGCTATCGGTCTCCGGCCAATATTTAGCTTTAGATGAAA14920.12280310431761558No Hit
CTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCT14770.12156848865758592No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT14490.11926387275886392No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGA14310.11778233396682834No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAT13820.11374925614406482No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGCCAACACCACTT13780.11342002530139025No Hit
CTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTA13650.1123500250626979No Hit
GCCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCCACGGCTGGC13590.11185617879868603No Hit
TCGCTACACGGCGCACGAGGGCGCCTTTAAAACGGAACTTCCCTATAGCT13170.10839925495060303No Hit
CCTTTGCAGGGTCCGGTGCGCCCCCGACGATCCTTGAAAATCCGCGGGAA12780.10518925423452594No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTT12490.10280233062513529No Hit
GGTTAATATTCCGGCACCTGGATGTGGATTCTCCACGGCAACGTAACTGA12470.10263771520379801No Hit
TCCTTATCCCGAAGTTACGGATCCATTTTGCCGACTTCCCTTATCTACAT12440.10239079207179208No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAAC12350.10165002267577428No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAA12160.10008617617307007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)