FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001866719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001866719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803858
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173230.45540606405391576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98830.25981516660190784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77080.2026363760161394No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA66430.17463848545345279No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG60200.1582603767017591No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG59970.15765572742200154No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT58870.15476392651881327No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG53590.14088328218350948No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50450.1326285050598629Clontech SMART CDS Primer II A (96% over 26bp)
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC48710.12805420181300145No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT48210.12673974685700676No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT47860.1258196283878105No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA47580.12508353361245345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC42160.11083484188947115No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG41490.1090734722484383No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41190.1082847992748415Clontech Universal Primer Mix Long (96% over 26bp)
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA41010.1078115954906834No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC40290.10591878035405107Clontech Universal Primer Mix Long (96% over 25bp)
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC40030.10523526377693383No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT39940.10499866188485481No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA39120.10284295575702351No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA38150.10029291314239386No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA38040.10000373305207502No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)