FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001867531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001867531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739591
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA464876.285501040439918Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT357114.828479524493943Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT336244.5462965341655055Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG200122.7058198382619585Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC157812.133746895243452Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG133301.802347513693379Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA131111.7727365530408021Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC129991.75759304804953Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG127481.723655371685161Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC105951.4325485302011518Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG83101.123593986406Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT78651.0634255960388919Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC75021.0143444146832505Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT71020.96026046828585Clontech Universal Primer Mix Long (100% over 23bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA64970.8784584993597813Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG62100.8396532678196462Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC61660.8337040337159322Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC61620.8331631942519582Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC58490.7908425061959922Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA56670.7662343105851748Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG53690.7259417705191112Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA53520.7236432027972217Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC50850.6875421685769567Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT49180.6649621209560419Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC45700.6179090875903033Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG40880.5527379321814354Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40420.5465182783457343Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT39940.5400282047780463Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT39490.5339437608083386Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG38990.5271832675086635Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC38630.5223157123328975Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA38550.5212340334049494Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC36530.4939216404742621Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG36170.4890540852984961Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT35930.485809048514652Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33400.45160095241829606No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31500.4259110778795307Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG31010.41928579444584907Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30150.407657745970408Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT29900.40427749932057044Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC29720.4018437217326874Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA29680.40130288226871336Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC29300.39616490736096033Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA28550.3860241674114477Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT27040.3656074776464289Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA26590.35952303367672134Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG26370.35654841662486425Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA25500.3447851582834296Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT25140.33991760310766356Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG23260.31449814830088524Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA22860.30908975366114516Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20920.2828590396584058Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA19810.2678507445331271Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC18670.2524368198098679Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA18440.24932699289201737Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG17800.24067356146843322Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT17770.24026793187045273Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA15760.21309074880575887Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA15740.21282032907377185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC15610.21106260081585632Clontech Universal Primer Mix Long (96% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC15310.20700630483605126Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG14510.19618951555657113Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC13910.18807692359696102Clontech Universal Primer Mix Long (96% over 25bp)
TGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC13880.18767129399898053Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT13560.18334457828718848Clontech SMART CDS Primer II A (96% over 26bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA13420.18145164016327942Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.17685450471950037No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA11740.15873638267637113Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10880.14710833420092997Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10720.14494497634503395Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.1406182606332419No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG10150.13723801398340435Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9840.1330465081376058Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT9810.13264087853962528Clontech SMART CDS Primer II A (96% over 26bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA9490.12831416282783323Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT9180.12412265698203467Clontech Universal Primer Mix Long (96% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACT8920.12060720046620363Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8840.1195255215382556No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG8660.11709174395037258Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT8370.11317065783656101Clontech Universal Primer Mix Long (96% over 26bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG8020.10843831252678846Clontech Universal Primer Mix Long (96% over 26bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7690.1039763869490029Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTG7690.1039763869490029Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT7570.10235386855708087Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)