FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001867566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001867566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743890
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61620.828348277299063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49190.6612536799795669Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT32850.44159754802457357Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG27040.36349460269663525Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA25130.3378187635268655Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC23570.3168479210635981Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG22560.30327064485340577Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG18510.24882711153530762Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC16540.2223447015015661Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC15520.20863299681404507Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT14760.19841643253706867Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG14700.19760986167309683No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14050.1888720106467354No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12660.17018645229805482No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG12610.16951430991141164Clontech SMART CDS Primer II A (96% over 26bp)
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG11580.155668176746562No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10660.14330075683232738Clontech Universal Primer Mix Long (96% over 26bp)
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10640.14303189987767007No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA10610.14262861444568417No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10540.1416876151043837No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10450.14047775880842597Clontech Universal Primer Mix Long (96% over 26bp)
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC9890.13294976407802228No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA9760.13120219387275Clontech Universal Primer Mix Long (96% over 25bp)
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT9650.12972348062213498No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC9260.12448077000631813No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC8660.11641506136659989No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC8640.11614620441194262Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG8380.11265106400139807Clontech Universal Primer Mix Long (96% over 26bp)
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT8260.1110379222734544No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC8170.10982806597749667Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7970.10713949643092392No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG7910.10633292556695209No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC7890.10606406861229482No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT7850.10552635470298029No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7830.105257497748323No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC7810.10498864079366572No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT7790.10471978383900846No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA7730.10391321297503664Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC7710.10364435602037934Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC7680.10324107058839345Clontech Universal Primer Mix Long (96% over 26bp)
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG7590.1020312142924357No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7530.10122464342846389No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)