FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001867707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001867707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1462723
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA324342.2173712999658854Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT318002.174027481621606Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA189311.2942300080056168Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT151081.032868150702491Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC133390.9119293263317798Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG123880.8469135988153601Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG116770.7983056258772168Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG102620.7015682395094629Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC92390.6316301856195602Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC90720.620213123058843Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGA87540.5984728482426269Clontech Universal Primer Mix Long (96% over 27bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT73550.5028293121800915Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG66090.45182854169928277Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA60890.4162784067796842Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGG57970.396315638709448Clontech Universal Primer Mix Long (96% over 27bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG54990.375942676774755Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT53360.3647990767903424Clontech Universal Primer Mix Long (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52080.35604827434859504No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50190.3431271676182025Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGC49510.3384783038210242Clontech Universal Primer Mix Long (96% over 27bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC49120.3358120437020543Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48510.33164173941340913No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC47270.3231643995479664Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG46240.3161227382081228Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT44220.3023128781047403Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGT42150.288161189780977Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC40510.2769492241524882Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40510.2769492241524882Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC39490.2699759284567208Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA38920.2660790867443802Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC38880.26580562416807557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAAC38620.26402811742209564Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG38470.26300263276095337Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT38410.26259243889649647Clontech Universal Primer Mix Long (100% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38150.26081493215051654No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC36240.24775709413197167Clontech SMART CDS Primer II A (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC35690.24399698370778333Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCG33440.22861471379064935Clontech Universal Primer Mix Long (96% over 27bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG31470.21514668190764757Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA30940.21152330277161158Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA30910.21131820583938313Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA29420.20113172487203662Clontech SMART CDS Primer II A (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA28840.1971665175156198Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC28570.19532064512556377Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT27940.19101360954876626Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27640.1889626402264817No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT27320.18677493961604488Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATG25990.17768230895391676Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG25880.1769302868690791Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG25770.17617826478424145Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA25120.17173449791929163Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC24370.1666070746135803Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT24020.164214277070915Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG23130.15812973474813755Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG23100.1579246378159091Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACAT23020.1573777126632999Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC22790.1558053028495484Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG22730.15539510898509148Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATA22180.15163499856090318Clontech Universal Primer Mix Long (96% over 27bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA22050.1507462451879132Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC21890.1496523948826948Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCT21530.14719123169595336Clontech Universal Primer Mix Long (96% over 27bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT20860.14261073354285125Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20450.13980774213572905Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG18980.12975799245653485Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.1285274108631641No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC18580.1270233666934888Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA18350.1254509568797373Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA17460.11936641455695986Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGT17310.1183409298958176Clontech Universal Primer Mix Long (96% over 27bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTG17170.11738381087875148Clontech Universal Primer Mix Long (96% over 26bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG16780.11471755075978159No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG16620.11362370045456317Clontech Universal Primer Mix Long (96% over 26bp)
TGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA16480.11266658143749704Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACA16470.11259821579342091Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCA16470.11259821579342091Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG16440.11239311886119246Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.1092482992336895No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG15790.10794935199624262Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTA15550.106308576538415Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT15530.10617184525026269Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT15120.1033688538431405Clontech Universal Primer Mix Long (96% over 27bp)
GAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG14930.10206990660569364Clontech Universal Primer Mix Long (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)