FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001868382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001868382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945869
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44860.23053967147839857No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA37780.1941548994305372No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTA35380.18182107839736386No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT33670.17303323091122783No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAA33330.17128593959819494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33000.1695900392061336No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG31890.16388564697829094No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGC31530.16203557382331493No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG31300.16085358264096913No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGT31210.16039106435222517No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTC31030.15946602777473715No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAA29020.14913645265945447No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGA28180.1448196152978438No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCC28040.14410014240424202No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGA27430.1409652962249771No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTT27270.1401430414894322No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGC26270.13500394939227667No Hit
GTATTGGTAGTACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCG24580.12631888374808375No Hit
TTTCTACCCTAGCGGCGAGGTATTGGTAGTACGCTTGAGCGCCATCCATT24560.12621610190614063No Hit
ACCAATACCTCGCCGCTAGGGTAGAAACGATGCCCTAGCGAGTAGGCAGG24520.12601053822225441No Hit
GTTATAGCCTCTTGTGATGCAGCGAGCGCCGGGCGAGGTCCGCGCTTCGG24390.1253424562496242No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACT24020.12344099217367664No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTT23290.11968945494275308No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGA23260.11953528217983841No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGG23250.11948389125886687No Hit
ATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAAT22440.11532122666017086No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATAAGCGTACATTCCGG21790.11198081679701974No Hit
GTCTAACATCTATGCGAGTGTTCGGGTGTCAAACCCCTACGCGTAATGAA21530.11064465285175928No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCA21170.1087945796967833No Hit
GCTCTATACGTAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTC20930.10756119759346594No Hit
GGATAAGACTTGAGGAATGTAGCTCCCTCGGGAGTGTTATAGCCTCTTGT20150.10355270575768462No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAA20120.10339853299476995No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGG19930.10242210549631038No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGAT19840.1019595872075664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19780.10165124168173706No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGT19780.10165124168173706No Hit
GGCTATAACACTCCCGAGGGAGCTACATTCCTCAAGTCTTATCCAGCGGC19590.10067481418327749No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)