FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001868423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001868423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315584
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91200.6932282545242265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53760.4086398131932283No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA39420.2996387915936953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATTAAGGGGGCGATTTGCCCCGCCCATCGAAAGGGAAAAGGGTTAAGA38210.29044135532204707No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT29480.22408299280015567Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29380.22332287410001947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28380.21572168709865733No Hit
GTACATGGGTTCGACCTGAGATCAGGCAAGGCTACCCGCTGAACTTAAGC25950.19725080268534734No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.15111159758707918No Hit
TATCTACATTGTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATG17620.13393291496400078No Hit
CCCCTAGACCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCC17620.13393291496400078No Hit
GTATCAACGCAGAGTACATGGGTTCGACCTGAGATCAGGCAAGGCTACCC17330.13172857073360578No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG17140.13028434520334697Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16750.1273198822728157No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA15920.12101089706168515No Hit
ATCCTAAGAGATGGGCAAATGCTGTATTAAGGGGGCGATTTGCCCCGCCC15750.1197186952714536No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC15730.11956667153142635Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT15460.11751435104105858Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTAGATGGGACTTACCACCAAATTAGGGCTGCATTCCCAAACAACCC14840.11280161510021404No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA14540.1105212589998054Clontech SMART CDS Primer II A (96% over 26bp)
TCAATAAGCGGAGGAAAAGAAACTAACCAGGATTCCCCTAGTAACGGCGA14100.10717673671920608No Hit
AATTAGGGCTGCATTCCCAAACAACCCGACTCTGCGAAAGCATCTCGTGG13660.10383221443860673No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCTG13490.10254001264837517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)