FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001868424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001868424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315584
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43850.3333120500097295No Hit
GTATTAAGGGGGCGATTTGCCCCGCCCATCGAAAGGGAAAAGGGTTAAGA37090.2819280258805215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36870.28025576474022185Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27940.212377164818058No Hit
GTACATGGGTTCGACCTGAGATCAGGCAAGGCTACCCGCTGAACTTAAGC25770.19588258902510217No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25000.1900296750340533Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19950.15164368067717454No Hit
GTATCAACGCAGAGTACATGGGTTCGACCTGAGATCAGGCAAGGCTACCC17230.13096845203346955No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16340.12420339560225725Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCGACTAGAGGCTGTTCACCTTGGAGACCTGATG16010.12169500389180773No Hit
CCCCTAGACCCAGCCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCC15750.1197186952714536No Hit
ATCCTAAGAGATGGGCAAATGCTGTATTAAGGGGGCGATTTGCCCCGCCC15210.11561405429071803No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC14450.10983715216968282Clontech Universal Primer Mix Long (96% over 25bp)
TCAATAAGCGGAGGAAAAGAAACTAACCAGGATTCCCCTAGTAACGGCGA13900.10565649931893364No Hit
GTCTTAGATGGGACTTACCACCAAATTAGGGCTGCATTCCCAAACAACCC13870.10542846370889278No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA13420.10200792955827982No Hit
GTATTTAGTCTTAGATGGGACTTACCACCAAATTAGGGCTGCATTCCCAA13380.10170388207822534No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA13230.10056370402802102Clontech SMART CDS Primer II A (96% over 26bp)
AATTAGGGCTGCATTCCCAAACAACCCGACTCTGCGAAAGCATCTCGTGG13170.10010763280793929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.10003162093792566No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)