FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001868882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001868882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861847
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG26320.30539063197992217No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG24360.28264877640694924No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22320.25897868183099787No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA19190.22266133083946454No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA18870.2189483748275506No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG17950.20827362629329801No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA17880.2074614171656918No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC17430.20224007277393785No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC17210.19968741551574698No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC17060.19794696738516232No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT16490.1913332644889406No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16330.18947678648298363No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT15960.18518368109420813No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14590.16928758816820155No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT14460.1677791997883615No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA13660.15849680975857663No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA13450.1560601823757581No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG13390.15536400312352425No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA13290.15420370436980113No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA13240.1536235549929396No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT13020.15107089773474874No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG13000.15083883798400413No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC12910.14979456910565334No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA12730.14770603134895172No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG12710.1474739715982071No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG12650.14677779234597324No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC12640.14666176247060092No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA12460.14457322471389936No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG12290.14260071683257006No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC12290.14260071683257006No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11830.13726334256544376No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTG11570.13424656580576366No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT11390.13215802804906207No Hit
GTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCG11290.13099772929533895No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT11230.1303015500431051No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG11110.12890919153863736No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG11030.1279809525356589No Hit
TATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGG11010.12774889278491425No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG10660.12368784714688338No Hit
TATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC10640.12345578739613874No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT10500.1218313691409264No Hit
ACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAG10410.12078710026257561No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAA10160.11788635337826783No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA10120.1174222338767786No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT10010.11614590524768317No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG9900.11486957661858775No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG9680.11231691936039691No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT9620.11162074010816304No Hit
CTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTA9130.10593527621491981No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG9070.10523909696268595No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG9010.10454291771045207No Hit
GGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATA8900.10326658908135666No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC8880.10303452933061205No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC8860.10280246957986743No Hit
CTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAG8780.10187423057688894No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC8660.10048187207242121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)