FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001868889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001868889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856419
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29340.3425893166779345No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA28520.33301456413274344Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24710.28852699438008733Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.26062009366910355No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15610.1822705941834546No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA15570.18180353308368918No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG15230.17783351373568312No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14640.1709443625141432No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC14180.16557315986684087No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGA13200.1541301629225881No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12980.15156132687387833No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT12850.1500433782996407No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12780.14922602137505123Clontech Universal Primer Mix Long (96% over 26bp)
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC12560.14665718532634142No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11740.13708243278115034No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT11680.1363818411315022No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACG11470.1339297703577338No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11410.13322917870808565Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC11140.13007651628466907No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10780.1258729663867803No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC10430.1217861817638329No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10160.11863351934041631Clontech Universal Primer Mix Long (96% over 26bp)
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10050.11734910131606141No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA9510.11104377646922828No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA9500.11092701119428691No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA9330.10894200152028388No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG9300.10859170569545983No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC9250.10800787932075304No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9240.10789111404581168No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT9220.10765758349592898No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9140.10672346129639815Clontech SMART CDS Primer II A (96% over 26bp)
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG9010.10520551272216053No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA8910.10403785997274699No Hit
ATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAA8810.10287020722333344No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT8750.10216961557368533No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8730.1019360850238026No Hit
CTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCAT8700.10158578919897854No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8670.10123549337415448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)