FastQCFastQC Report
Fri 30 Dec 2022
EGAF00001878169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246558558
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT8175510.3315849210961073TruSeq Adapter, Index 25 (97% over 44bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA6425170.2605940776146168No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT5848880.2372207254716342No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG5415570.21964640140375902No Hit
ATTTTTTATTAATTGAAGCACAGAGAAAAGAGGCAAAATGATTAAAGAAA5103210.20697760570127932No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC4419960.17926613603896888No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG3943200.1599295531246577No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT3880810.1573991197661044No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC3335830.13529564850878142No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT3222050.13068092327178518No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA3072490.12461502147493903No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC2724250.11049099338097199No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT2659870.10787984897283509No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA2537530.10291794454768022No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)