FastQCFastQC Report
Mon 18 Sep 2023
EGAF00001878225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41476874
Sequences flagged as poor quality0
Sequence length101
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG7590901.8301523880512305TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC4095050.9873092171796747TruSeq Adapter, Index 5 (100% over 50bp)
TGGTGGTTGTTGTATTATTAGATCGGAAGAGCACACGTCTGAACTCCAGT550200.13265223411002477Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)