FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001878249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12643551
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA5363114.241775115234637No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3950503.1245177877638963RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA3569642.823289121861414No Hit
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG3379782.673125611626038RNA PCR Primer, Index 1 (100% over 29bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA3197652.5290758901514296No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG3113122.4622196723056677RNA PCR Primer, Index 1 (100% over 29bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG2428771.9209555923015615RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA1846881.460728872766836RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1644611.3007500819983246RNA PCR Primer, Index 1 (100% over 29bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG1624021.2844651000340015No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG1493701.181392790680403No Hit
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1400311.1075290478125963RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1081580.8554400579394191RNA PCR Primer, Index 1 (100% over 28bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC1009370.7983279380927083RNA PCR Primer, Index 16 (100% over 51bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCCA956750.7567098831649431RNA PCR Primer, Index 1 (100% over 28bp)
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC918230.7262437585770011No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT916920.7252076572475565No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC879870.6959041807163193RNA PCR Primer, Index 1 (100% over 31bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC842610.6664346116055528No Hit
TGGAGTGTGACAATGGTGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA840490.6647578674693525RNA PCR Primer, Index 1 (100% over 28bp)
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC768300.6076615659635494No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC714110.5648017712745415No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGACTTGGAATTCTCGGGTGCCAA691720.5470931386285387No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT630520.49868901545143446RNA PCR Primer, Index 1 (100% over 30bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG603840.4775873486807622No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT557650.4410548903547746No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCAG552380.436886757525635RNA PCR Primer, Index 1 (100% over 29bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAAATGTGTGCTGATTGTCA549350.4344902788781411No Hit
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG543400.4297843224581449RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTGTGGAATTATCGGGTGCCAAGGAACTCCAG540900.42780702984470104RNA PCR Primer, Index 1 (96% over 29bp)
TCAGTGCACTACAGAACTTTGTTGGAATTATCGGGTGCCAAGGAACTCCAG536470.4243032673336786RNA PCR Primer, Index 1 (96% over 29bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC498700.39443033052976967No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA498670.3944066030184084No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA497820.3937343235298375No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG472090.3733840279522739RNA PCR Primer, Index 7 (97% over 36bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC458950.3629913779760132RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG449170.35525620927222107RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT444210.3513332607271486RNA PCR Primer, Index 1 (100% over 30bp)
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC419430.3317343363426936No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG386670.305823893936126RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC378180.2991090082208709No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC362310.2865571547107296RNA PCR Primer, Index 1 (100% over 27bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC354310.28022981834770944RNA PCR Primer, Index 2 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG352900.2791146253137271No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT324670.2567870371227197No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTATCGGGTGCCAAGGAACTCCAG318910.2522313549413452RNA PCR Primer, Index 1 (96% over 29bp)
CACAGATGATGAACTTATTGACGGGCGGAAAGAAACTGTGTGCTGATTGTC301750.23865921844266696No Hit
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACTCC300450.23763102628367613RNA PCR Primer, Index 1 (100% over 27bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCAG299750.2370773843519119RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC296940.23485490745440105RNA PCR Primer, Index 1 (100% over 27bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA294230.23271152226142797RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA292610.23143023664791643No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA289000.22857502611410355RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG278950.22062630980805945RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG278070.21993030280812723RNA PCR Primer, Index 7 (97% over 36bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA274870.21739936826291917RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTATCGGGTGCCAAGGAACTCCAG271970.21510570883132435RNA PCR Primer, Index 1 (96% over 29bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC265640.21009920393408466No Hit
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG252190.19946136967375702RNA PCR Primer, Index 1 (100% over 29bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTATCGGGTGCCAAGGAACTCCAG248130.1962502464695243RNA PCR Primer, Index 1 (96% over 29bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA247000.19535651020824765RNA PCR Primer, Index 1 (100% over 23bp)
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT239850.1897014533337984No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA238520.18864953366344628No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG232650.18400685060708025Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC226780.1793641675507142RNA PCR Primer, Index 16 (100% over 44bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG223810.17701514392594295Illumina Small RNA Adapter 2 (100% over 21bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC217150.17174763640372867RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC216060.17088553682426716RNA PCR Primer, Index 1 (100% over 27bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG212840.16833878393815158RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATGGAATTCTCGGGTGCCAA207170.16385428429086102No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA198540.15702867018925298RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCCA196630.15551801863258194No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCATTGGAATTCTCGGGTGCCAAG192460.1522198945533577No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC190540.15070133382623283No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC185990.14710266126976512RNA PCR Primer, Index 1 (100% over 27bp)
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATACT185490.14670720274707635No Hit
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC178610.141265693474879RNA PCR Primer, Index 5 (97% over 35bp)
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACTCC176790.13982622445229193RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTAGGGTGCCAAGGAACTCCAGTC173660.1373506541002603RNA PCR Primer, Index 1 (96% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA172890.1367416479753196No Hit
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG170960.13521517807774097RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCCA170840.13512026803229568No Hit
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCC168320.1331271570779443No Hit
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC168040.1329057003052386RNA PCR Primer, Index 1 (100% over 31bp)
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATGA159230.12593772113546267No Hit
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT156960.12414233944245567RNA PCR Primer, Index 1 (100% over 30bp)
CATTATTACTTTTGGTACGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGT156380.12368360755613671RNA PCR Primer, Index 1 (100% over 30bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATT154130.1219040442040373No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCAAGT153540.12143740314726457RNA PCR Primer, Index 1 (96% over 30bp)
TGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGT152320.12047248435190398RNA PCR Primer, Index 1 (100% over 30bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGAC150060.11868501182935079No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC149190.11799691399987235No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG146130.11557670784101715RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCAA141270.11173285100048239No Hit
CTGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCA141150.11163794095503708RNA PCR Primer, Index 1 (100% over 28bp)
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC139320.11019056276199621RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCAA138620.10963692083023194No Hit
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG132120.10449596003527806RNA PCR Primer, Index 1 (100% over 29bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC131580.1040688648307742RNA PCR Primer, Index 1 (100% over 24bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG129910.10274803336499375RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)