FastQCFastQC Report
Fri 23 Dec 2022
EGAF00001878261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191623534
Sequences flagged as poor quality0
Sequence length101
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC49843172.601098568613185Illumina Single End PCR Primer 1 (100% over 50bp)
AATAATACAACAACCACCAAAATCTACACTCTTTCCCTACACAACACTCT2217780.11573630616790526Illumina Single End PCR Primer 1 (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)