FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001878278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10123914
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG5305265.240325036344639RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT4649164.592255524888892RNA PCR Primer, Index 1 (100% over 30bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA4529854.474405847382742No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG2872102.8369462640634837No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA2384502.355314357668388No Hit
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC2330112.3015900767232913No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2115702.0898043977852834RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA1882931.859883440337403RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1813411.7912143465462074RNA PCR Primer, Index 1 (100% over 29bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1783961.7621248066706217RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1606331.5866689503684048RNA PCR Primer, Index 1 (100% over 28bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1431651.4141269868550839RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA1393941.3768785471705904No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC1279471.2638096293587637No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG881080.8702958164204081RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT863050.8524864987987848No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTCGTATGCC812820.8028713005661645RNA PCR Primer, Index 15 (100% over 51bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCAG810750.8008266368126004RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA797800.788035141349482RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC779260.7697220659914733RNA PCR Primer, Index 1 (100% over 31bp)
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG757830.7485543634606142RNA PCR Primer, Index 1 (100% over 29bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC755260.746015819573339No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT695800.6872835940724111No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAAATGTGTGCTGATTGTCA691730.6832634097840025No Hit
TGGAGTGTGACAATGGTGTTTGTGGAATTATCGGGTGCCAAGGAACTCCAG677440.6691483155625384RNA PCR Primer, Index 1 (96% over 29bp)
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC591420.5841811773588753No Hit
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC570050.5630727404440615No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT566520.5595859467000608RNA PCR Primer, Index 1 (100% over 30bp)
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT523460.5170529895848582No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC467660.4619359666626959No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA458940.45332269713077367RNA PCR Primer, Index 1 (100% over 28bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCAG422100.41693360887893754RNA PCR Primer, Index 1 (100% over 29bp)
CACAGATGATGAACTTATTGACGGGCGGAAAGAAACTGTGTGCTGATTGTC417260.41215284918461376No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG411190.4061571443613607RNA PCR Primer, Index 1 (100% over 29bp)
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT397270.3924075214388427No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC394240.3894146078285533RNA PCR Primer, Index 1 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGCC388110.38335963738925477No Hit
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA372230.36767400434258923RNA PCR Primer, Index 1 (100% over 34bp)
TGGAGTGTGACAATGGTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT362760.35831991461010043RNA PCR Primer, Index 1 (100% over 30bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA346890.3426441591661091RNA PCR Primer, Index 1 (100% over 23bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA344750.34053035219382544RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA334310.330218135001937No Hit
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACTCC303670.29995316040811887RNA PCR Primer, Index 1 (100% over 27bp)
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATGA291470.2879024851455672No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTATCGGGTGCCAAGGAACTCCAG290030.28648011036047916RNA PCR Primer, Index 1 (96% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG271000.2676830324714335No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC270170.26686319144947296RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC268410.2651247333788098No Hit
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACTCC257570.25441741207995244RNA PCR Primer, Index 1 (100% over 27bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTATCGGGTGCCAAGGAACTCCAG251900.24881681136366823RNA PCR Primer, Index 1 (96% over 29bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTATCGGGTGCCAAGGAACTCCAG237070.23416832659779607RNA PCR Primer, Index 1 (96% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG234460.23159027229982398No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGACTTGGAATTCTCGGGTGCCAA223020.22029029484051327No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGAC222100.21938155539448478No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC218270.2155984335702575RNA PCR Primer, Index 1 (100% over 27bp)
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCC217460.21479834775364548No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC212950.21034354894757107RNA PCR Primer, Index 1 (100% over 27bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC212590.20998795525129904RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC210550.20797292430575764RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG206350.2038243311825841RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA199540.19709768376143852No Hit
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG192040.18968948175577155RNA PCR Primer, Index 15 (100% over 36bp)
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATACT189090.18677558896687585No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCCA180670.1784586475151804No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTTGGAATTCTCGGGTGCCA179060.17686835348463056No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG178450.176265819721503RNA PCR Primer, Index 1 (100% over 29bp)
GGCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAA172260.17015158366615915No Hit
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG169790.16771181580562616RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC165210.16318787378083222No Hit
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC163820.16181488700911525RNA PCR Primer, Index 1 (100% over 31bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA162750.16075798352297344RNA PCR Primer, Index 1 (100% over 22bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG162630.16063945229088275RNA PCR Primer, Index 1 (100% over 29bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGCC159380.15742923142176038No Hit
GGTCATGATGTCAAAACTAAGTTCTGACTTGGAATTCTCGGGTGCCAAGGA158340.15640196074364124RNA PCR Primer, Index 1 (100% over 22bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT146150.14436116308376384RNA PCR Primer, Index 1 (100% over 35bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG144460.1426918482318202RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG143980.14221772330345753RNA PCR Primer, Index 1 (100% over 29bp)
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA142390.14064718447825614RNA PCR Primer, Index 1 (100% over 28bp)
GGGCGGTGATGACCCCAACATGCCATCTGAGTGTCGGTGCTGAAATCCAGA140510.13879019517550228No Hit
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG138910.13720977874762666RNA PCR Primer, Index 1 (100% over 29bp)
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCAAGT138350.1366566329978702RNA PCR Primer, Index 1 (96% over 30bp)
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT137670.13598495601602306RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGTCAATCTC136590.134918174927207RNA PCR Primer, Index 15 (100% over 44bp)
ACAGATGATGAACTTATTGACGGGCGGAAAGAAACTGTGTGCTGATTGTCA135060.13340690171805095No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC134380.1327352247362038RNA PCR Primer, Index 1 (100% over 27bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC133120.13149064679925176RNA PCR Primer, Index 1 (100% over 24bp)
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCA132670.13104615467891173No Hit
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC127760.1261962517658684No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG124830.12330211418232118RNA PCR Primer, Index 15 (100% over 36bp)
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG121560.12007213810785039RNA PCR Primer, Index 1 (100% over 29bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTCTC120700.11922266427786724No Hit
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC119640.11817563839439964RNA PCR Primer, Index 1 (100% over 31bp)
TCTTAACCCGGACCAGAAACTATGGAATTATCGGGTGCCAAGGAACTCCAG118770.11731628696174227RNA PCR Primer, Index 1 (96% over 29bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTAGGGTGCCAAGGAACTCCAGTC116580.11515309197608752RNA PCR Primer, Index 1 (96% over 31bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA112750.11136997015186025No Hit
AGAAATGAAGAAACTAAAATTGGTCTTAGTATTGAAGTGAAGACACTGAGA112170.11079706919675533No Hit
CTGACCTATGAATTGACAGCCATTGGAATTCTCGGGTGCCAAGGAACTCCA108580.1072510098367094RNA PCR Primer, Index 1 (100% over 28bp)
ACAAATGATGAATAACAAAGGGACTTAATACTGAAACCTGATGTGGAATTC108420.10709296819392183No Hit
GACACAAGGGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGT106470.10516683567244843RNA PCR Primer, Index 15 (100% over 37bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA104150.10287523185202878RNA PCR Primer, Index 1 (100% over 34bp)
TCAGTGCACTACAGAACTTTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA103400.10213441165146206RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA103100.10183808357123539RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)