FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001878281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12135940
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG5632694.641329802223808RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA4253623.504977776752357No Hit
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTCA4033063.3232366013675083No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGT3883843.2002795003930475RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG3113532.5655449845665026RNA PCR Primer, Index 1 (100% over 29bp)
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATG3021182.4894486953626997No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCA2780212.29088970446459No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG2496042.0567339653953463RNA PCR Primer, Index 1 (100% over 29bp)
TCAGTGCACTACAGAACTTTGTCTGGAATTCTCGGGTGCCAAGGAACTCCA2238991.8449250737890925RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAG1519261.2518684172795844RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTGATGGAATTCTCGGGTGCCAAGGAACTCCA1434631.1821333988137714RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG1429391.1778156451004207RNA PCR Primer, Index 1 (100% over 29bp)
TGACCTATGAATTGACAGCCAGTTGGAATTCTCGGGTGCCAAGGAACTCCA1381861.1386509821241702RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC1379721.1368876246916184RNA PCR Primer, Index 1 (100% over 31bp)
AGTGATGATGACCCCAGGTAACTCTGAGTGTGTCGCTGATGCCATCACCGC1298121.0696493225905863No Hit
CACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC1098240.9049484423950679No Hit
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCTC874440.7205375108973842No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG859440.7081775288935179RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTGTGGAATTATCGGGTGCCAAGGAACTCCAG805960.6641100730557337RNA PCR Primer, Index 1 (96% over 29bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCC767040.6320400397497021RNA PCR Primer, Index 1 (100% over 27bp)
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAGT750220.6181803799293668RNA PCR Primer, Index 1 (100% over 30bp)
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT740480.6101546316148563No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGAT725100.5974815300668923No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAAG717350.5910955393648947No Hit
TGGAGTGTGACAATGGTGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA700280.5770298798444949RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGACTTGGAATTCTCGGGTGCCAA683620.5633020598322009No Hit
CTGACCTATGAATTGACAGCCTTGGAATTCTCGGGTGCCAAGGAACTCCAG651490.5368269783799196RNA PCR Primer, Index 1 (100% over 29bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAAATGTGTGCTGATTGTCA637680.52544755494836No Hit
CTGCTGTGATGACATTCCAATTAAAGCACGTGTTAGACTGCTGACGCGGGT630690.5196878033345583No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC623920.5141093314568134No Hit
TCAAGTGATGGAGAGCAATACCCGGGGATGACTGTGACCACATTGGGATGT514820.42421106234869327No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCCTGGAATTCTCGGGTGC507880.41849251067490445No Hit
GTGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATAC496730.40930492405203056No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA477120.3931463075789761RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC476800.3928826279628937RNA PCR Primer, Index 1 (100% over 27bp)
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCAG464320.382599122935677RNA PCR Primer, Index 1 (100% over 29bp)
TGGAGTGTGACAATGGTGTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT444330.36612738691852464RNA PCR Primer, Index 1 (100% over 30bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCCA436690.3598320360845555No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG436310.3595189165404575No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCAG398830.3286354415067972RNA PCR Primer, Index 1 (100% over 29bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCCAAG398340.32823168209467085No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCCA396510.32672376429019917No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTATCGGGTGCCAAGGAACTCCAG380760.31374578318613966RNA PCR Primer, Index 1 (96% over 29bp)
TTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACATGA375870.3097164290528793No Hit
CACAGATGATGAACTTATTGACGGGCGGAAAGAAACTGTGTGCTGATTGTC361020.2974800468690518No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG358000.29499157049227337RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTCGACTGGATGGAATTCTCGGGTGCCAAGGAACTCCAGTC357350.2944559712721058RNA PCR Primer, Index 1 (100% over 31bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGAA353680.29143189567515987RNA PCR Primer, Index 1 (100% over 23bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTATCGGGTGCCAAGGAACTCCAG349150.2876991811099923RNA PCR Primer, Index 1 (96% over 29bp)
AGTGATGATGACCCCAGGTAACTCTGAGTTGGAATTCTCGGGTGCCAAGGA343990.2834473473006623RNA PCR Primer, Index 1 (100% over 22bp)
GCTAATGATGGAAAAATCATTATTGGAAAAGAATGACATGAACAAAGGAAC333350.2746800000659199No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG321860.26521225385095837Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC321590.2649897741748888RNA PCR Primer, Index 1 (100% over 27bp)
TAGCATATCGAGCCTGAGAACATGGAATTCTCGGGTGCCAAGGAACTCCAG320690.2642481752546568RNA PCR Primer, Index 1 (100% over 29bp)
CAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTC307220.253148911415185No Hit
TGTAAACATCCTCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC305740.25192939319080354RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG291730.2403851699991925No Hit
AACCCGTAGATCCGAACTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT283770.23382613954914083RNA PCR Primer, Index 1 (100% over 30bp)
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGAC276150.22754726869117675No Hit
TCAGTGCACTACAGAACTTTGTCTTGGAATTCTCGGGTGCCAAGGAACTCC261480.21545920629139564RNA PCR Primer, Index 1 (100% over 27bp)
TGAAATGATGGCAATCATCTTTCGGGACTGACCTGAAATGAAGAGAATACT259900.2141572881869884No Hit
AACCCGTAGATCCGAACTTGTGATGGAATTCTCGGGTGCCAAGGAACTCCA248020.20436818243992635RNA PCR Primer, Index 1 (100% over 28bp)
AACCCGTAGATCCGAACTTGTGTGGAATTCTCGGGTGCCAAGGAACTCCAG242460.19978674911049332RNA PCR Primer, Index 1 (100% over 29bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTATCGGGTGCCAAGGAACTCCAG238430.19646603394545456RNA PCR Primer, Index 1 (96% over 29bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTCC235640.19416707729273547RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG225380.185712849602091RNA PCR Primer, Index 6 (100% over 34bp)
TTCAAGTAATTCAGGATAGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG223830.18443565146169147RNA PCR Primer, Index 1 (100% over 29bp)
GACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC223620.18426261171363734RNA PCR Primer, Index 6 (97% over 36bp)
TCAGTGCACTACAGAACTTTGTTTTGGAATTCTCGGGTGCCAAGGAACTCC218220.17981301819224552RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCC218100.17971413833621458RNA PCR Primer, Index 11 (100% over 51bp)
ACTGGACTTGGAGTCAGAAGGCTGGAATTCTCGGGTGCCAAGGAACTCCAG213230.17570126417895934RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG212840.17537990464685885Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAAC212500.1750997450547712RNA PCR Primer, Index 1 (100% over 24bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTATCGGGTGCCAAGGAACTCCAG209990.17303150806612425RNA PCR Primer, Index 1 (96% over 29bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGC209440.17257830872598248RNA PCR Primer, Index 6 (97% over 36bp)
GCAAATGATGATAAACTGGATCTGACTGACTGTGCTGAGTCTGTTCAATCC207280.17079847131742576No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTAGGGTGCCAAGGAACTCCAGTC203370.16757663600841796RNA PCR Primer, Index 1 (96% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC193020.15904824842575027No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG192400.1585373691695905RNA PCR Primer, Index 1 (100% over 29bp)
TGTAAACATCCTACACTCAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG187930.15485409453243837RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG176020.1450402688213686RNA PCR Primer, Index 6 (100% over 34bp)
GCATTTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA169290.13949475689563395No Hit
CTGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCAAGT167180.1377561194270901RNA PCR Primer, Index 1 (96% over 30bp)
TGAGAACTGAATTCCATAGGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA166160.13691564065082723RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCATGGAATTCTCGGGTGCCAA165380.13627292158662618No Hit
GGGCGGTGATGACCCCAACATGCCATCTGAGTGTCGGTGCTGAAATCCAGA165070.13601748195854627No Hit
TGACCTATGAATTGACAGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC164640.13566316247443544RNA PCR Primer, Index 1 (100% over 31bp)
CTGACCTATGAATTGACAGCCATGGAATTCTCGGGTGCCAAGGAACTCCAG157680.12992813082464152RNA PCR Primer, Index 1 (100% over 29bp)
ATTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA156190.12870037261225747RNA PCR Primer, Index 1 (100% over 22bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGG153390.1263931759715358RNA PCR Primer, Index 2 (97% over 35bp)
TGAGGTAGTAGGTTGTATGGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG151970.1252230976751698RNA PCR Primer, Index 1 (100% over 29bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGTG151080.12448973874294039No Hit
CTGACCTATGAATTGACAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC147660.1216716628460589RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA147340.12140798322997641RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCTC146190.12046038460968No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCCA143250.11803782813692223RNA PCR Primer, Index 1 (100% over 28bp)
TGGAGTGTGACAATGGTGTTTGTGGAATTCTCGGGTGCCAAGGAACTACAG142210.11718086938465418RNA PCR Primer, Index 1 (96% over 29bp)
TGAGGTAGTAGTTTGTACAGTTTGGAATTCTCGGGTGCCAAGGAACTCCAG140770.11599431111228302RNA PCR Primer, Index 1 (100% over 29bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA140440.11572239150819796RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCATGGAATTCTCGGGTGCCA139490.1149395926479531No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTC138210.11388487418362318RNA PCR Primer, Index 11 (100% over 44bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTTGGAATTCTCGGGTGCCA135970.11203911687104584No Hit
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTCC133540.1100367997864195RNA PCR Primer, Index 1 (100% over 27bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAAATCCAG129540.10674080458538852RNA PCR Primer, Index 1 (96% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA129280.10652656489732151No Hit
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTCC129070.1063535251492674RNA PCR Primer, Index 1 (100% over 27bp)
ACATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTTGGAATTC128970.10627112526924161No Hit
TTCACAGTGGCTAAGTTCTGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG127860.10535648660095552RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA125960.10379088888046578No Hit
GGCCAAGGATGAGAACTCTAATCTGATTTGGAATTCTCGGGTGCCAAGGAA124420.10252193072806887RNA PCR Primer, Index 1 (100% over 23bp)
ACAGATGATGAACTTATTGACGGGCGGAAAGAAACTGTGTGCTGATTGTCA123590.10183801172385493No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTATCGGGTGCCAAGGAACTCCAG122860.10123649259966677RNA PCR Primer, Index 1 (96% over 29bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCATTGGAATTCTCGGGTGCCAAG122090.10060201352346831No Hit
TGAGGTAGTAGGTTGTATAGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT121440.10006641430330078RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)