FastQCFastQC Report
Tue 24 Jan 2023
EGAF00001878609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136480471
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA2997680.21964168045697907No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT2210210.1619433156850697No Hit
CTCCATCTAAGGCTAAATACCGGCACGAGACCGATAGTCAACAAGTACCG2030830.1488000433409993No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1752810.12842936334825514No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1719440.12598432489289987No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1700380.12458778809460586Illumina Single End PCR Primer 1 (100% over 50bp)
GTGAGGCCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGT1659340.12158076447435472No Hit
CACGCCTTTGGCACAATGAAGTGGGTAACCTTTATTTCCCTTCTTTTTCT1500260.10992488441807911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)