FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001878702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26295679
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG6397212.4327989400844148TruSeq Adapter, Index 8 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG754270.2868418039328819TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGCCGGCTTCTGCTTGAAAAAAAAAAAG362090.13769942962872342TruSeq Adapter, Index 8 (98% over 52bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAAG330750.12578112168162683TruSeq Adapter, Index 8 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)