FastQCFastQC Report
Mon 18 Sep 2023
EGAF00001878704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63564274
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG39575796.2261058782799905TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1669110.262586181665506TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAAG1102180.1733961438779274TruSeq Adapter, Index 7 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1074940.169110717759476TruSeq Adapter, Index 7 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)