FastQCFastQC Report
Thu 23 Feb 2023
EGAF00001878786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001878786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114462896
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG1174934210.264760381390316TruSeq Adapter, Index 22 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAAACTCGTATG14183441.2391299273084966TruSeq Adapter, Index 22 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATATCGTATG11652661.018029458209759TruSeq Adapter, Index 22 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT6734980.5883985322195587TruSeq Adapter, Index 22 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAAAATCGTATG3223680.2816353694213713TruSeq Adapter, Index 22 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAAGCTCGTATG1704560.1489181262721153TruSeq Adapter, Index 22 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAACCTCGTATG1285090.11227131628750682TruSeq Adapter, Index 22 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)