FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001879161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001879161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4250157
Sequences flagged as poor quality0
Sequence length126
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT153790.3618454565325469No Hit
GGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCTCTGCTCCTAC107990.2540847314581555No Hit
CTCTGGTCCTGGAACTACTCCCAGCCCTGTTCCCACCACCAGCACAACCT93950.22105065765805829No Hit
GTTCCCACCACCAGCACAACCTCTGCTCCTACAACCAGCACAACCTCTGC82810.19483986120983296No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA78770.18533433000239757No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA55580.13077163973001468No Hit
GATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAG54730.12877171360963843No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG47980.11288994735959167No Hit
GCCGAGCGTCTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAA45790.10773719653179871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)