FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001879166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001879166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4946802
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTC167690.33898668270935445No Hit
GTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTG151360.3059754564666223No Hit
GTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATCT135690.2742984255282504No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGG130590.2639887345400119No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTG121370.2453504304397063No Hit
CTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAA111120.2246299730613839No Hit
GGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTT93250.1885056244418111No Hit
GCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACT86170.17419334754049182No Hit
ATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGG82370.1665116170002357No Hit
GGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCT81790.1653391423388282No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACT75920.15347289016216942No Hit
GCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCGGAAGG74320.1502384773031142No Hit
GGCGGACTTTGGAGTGGGAAGCGGGGAATGTCTACGTGCGTATGCACGTG73000.14757008669439367No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC72320.14619546122929522No Hit
CATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGAGCTGACGCTCG66130.1336823264808254No Hit
GGTGTCTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACG62570.12648575786942756No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA60550.12240231163487038No Hit
CTTTGATCTTCTTCCCCTTCTTTTCTTCCCCTTCCCCTTCTTTTCAATTT59610.12050209408017544No Hit
CTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTCT57100.11542810890753258No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGC56720.11465993585350698No Hit
CTTGATATTATCATCTGCTGAGGGTGGAGCTGAGGGTGGAAGGGGAGTGA51700.10451196550822126No Hit
GGTGGAAGCGGCCCCCGCAGACGCTCGGCAGGTGTCTTGATATTATCATC51490.10408744882047027No Hit
GGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCCGC51320.10374379245419567No Hit
GTGGAAGGGGAGTGAGCTGACGCTCGGAAGGTGTCTTGAGATTATCATCC50170.10141905821174974No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)