FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001879231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001879231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1490569
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62910.4220535916150141No Hit
TGTTATTCGACTCCTTCAGGTTATCTTGAACTGCCTGACTTAGGCCAGCC57470.38555746161365223No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44690.2998183915001587No Hit
CTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTCC44420.298007002694944No Hit
GCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCA42920.28794373155486264No Hit
CCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGG42480.28499183868710537No Hit
CTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCT37810.2536615212043186No Hit
TGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGCC36790.24681849682906326No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34250.22977802436519207No Hit
CAGCTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATAT33820.22689321997170206No Hit
AATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGC30830.20683376616580645No Hit
TCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCTC30270.20307681160684274No Hit
TGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGCC27560.184895835080429No Hit
GTTATCTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTAC26100.17510091783741646No Hit
CTTGAACTGACTGACTCATGCCAGCCCTACAGAAGTGCCTTTTATGTATT25180.1689287782048332No Hit
CACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGA24620.16517182364586946No Hit
GGAAGTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGA24310.163092080943586No Hit
CTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTCTGCGG24100.16168322298397456No Hit
TGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCT23800.1596705687559583No Hit
CTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTC22920.1537667830204439No Hit
CTCTCACATTGTCCTGTTCTCATGCTGAGGAGCCTGAGATCCCTGTGTGG22280.14947312066734247No Hit
GGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCAC22190.1488693243989376No Hit
ATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTGACTCCTGCCAGC21890.1468566701709213No Hit
ATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCATCATGCCCCAG21710.14564907763411156No Hit
CCTGAAGATGCCCAAGATGAAAATGCCCAAGTTCAGCATGCCTGGCTTCA20880.14008073426993317No Hit
GATGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACC20830.1397452918985971No Hit
CTCAGCTCCACCCTCAGCAGATGATAATATCAAGACACCTGCCGAGCGTC20690.13880605325885617No Hit
AGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATG20550.13786681461911526No Hit
GGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGACTCACTGGAT20380.1367263105565727No Hit
CTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCTTGAACAGCCTG20230.13571998344256456No Hit
ATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAGCT19790.13276809057480735No Hit
CACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACT19680.13203011735786804No Hit
CTCACTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAA19660.1318959404093336No Hit
TGATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCT19620.13162758651226478No Hit
GGTTGATCTTTGCTGGGAAACAGCTGGAAGATGGACGCACCCTGTCTGAC19420.1302858170269206No Hit
CATGGATGAAATTGAAAAGTACCAAGAAGTGGAAGAAGACCAAGACCCAT19380.13001746312985174No Hit
AGAAGATCAAAGAAGGAAAGAAGAAGGGGAAGAAAAGAAGGGGAAGAAGA19370.12995037465558454No Hit
GAAAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCGACTC18970.12726683568489616No Hit
GAAAGGGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC18970.12726683568489616No Hit
TAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACCCTCAG18400.12344279265166525No Hit
GCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGC18130.12163140384645059No Hit
CTGCGGGGGCCGCTTCCACCCTCAGCGGATGATAATCTCAAGACACCTTC17710.11881368792722778No Hit
GATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCACTCCCCTTCCACC17640.11834406860735731No Hit
ATGTTATTCAACTCCTTCAGGTTGTCTTGAACTGACTGACTCATGCCAGC17160.11512382184253127No Hit
AGTAGAGCCTGAAGTCTTGCAGGACTCACTGGATAGATGTTATTCAACTC17130.11492255641972965No Hit
GGTGAATTGGCTTATTTTGTCTGTCCCTGTCTGAATGTATTGCAGGAATT17110.11478837947119522No Hit
CCACCCTCAGCGGATGATAATCTCAAGACACCTTCCGAGCGTCAGCTCAC17030.11425167167705755No Hit
GATAATATCAAGACACCTGCCGAGCGTCTGCGGGGGCCGCTTCCACCCTC17020.11418458320279035No Hit
ATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGGATGAGAAA16730.11223901744904129No Hit
GGAAGAAGATCAAAACCCACCATGCCCCAGGCTCAGCAGGGAGCTGCTGG16640.11163522118063639No Hit
CTTGAACAGCCTGACTCCTGCCAGCCCTATGGAAGTTCCTTTTATGCATT16390.10995800932395615No Hit
CTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATCAAGACA16060.10774408967313825No Hit
CAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGATC15910.1067377625591301No Hit
GTCTTGCAGGACTCACTGGATAGATGTTATTCAACTCCTTCCAGTTGTCT15750.10566434697085475No Hit
CTTGAACTGCCTGACTTAGGCCAGCCCTACAGCAGTGCTGTTTACTCATT15670.10512763917671708No Hit
GGCAAAGATCCAAGACAAGGAAGGCATCCCTCCTGACCAGCAGAGGTTGA15440.10358460426857126No Hit
GGCTCAGCAGGGAGCTGCTGGAGGTAGTAGAGCCTGAAGTCTTGCAGGAC15390.10324916189723521No Hit
AGAAGGGGAAGAAAAGAAGGGGAAGAAGATCAAAACCCACCATGCCCCAG15260.10237701173176149No Hit
GTTCCTTTTATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTG15240.10224283478322707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)