FastQCFastQC Report
Tue 14 Feb 2023
EGAF00001879376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001879376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101819291
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTG7377620.7245797851803937No Hit
GGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCC5690030.5588361443216099No Hit
CTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC4863080.4776187255124375No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGG4464570.4384797768823592No Hit
CTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGGGCTGCCTG4404140.43254475225131944No Hit
AGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCC3983720.39125395206297403No Hit
GCTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGT3799770.37318763101581603No Hit
AAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCC3543990.34806665467745207No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3132040.307607720426967No Hit
TGCTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGG2886640.28350619726864923No Hit
ATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTG2866060.2814849692874015No Hit
GCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCC2722190.2673550339296705No Hit
CAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGA2668670.26209866261983694No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG2346700.23047695352740183No Hit
AGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGAT2175860.21369820773943518No Hit
CGCTGGTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGG2067440.2030499308819583No Hit
CAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG2020760.19846533796822452No Hit
GAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATG1888020.18542851570239277No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT1867020.1833660381705074No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1637660.1608398549936868TruSeq Adapter, Index 6 (100% over 50bp)
CTCGGTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCTAGT1568630.15406019670673213No Hit
CAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCC1559330.1531468137997543No Hit
GGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCT1529340.15020139945779037No Hit
GGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGGGCTGC1517980.14908569732625618No Hit
CATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCT1392920.13680315255779968No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA1382890.1358180740032849No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGT1336600.1312717842437147No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC1289230.12661942421107608No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC1282880.12599577029072026No Hit
GGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGC1266830.12441944817706499No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT1205240.11837049621569255No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG1172560.11516088832321568No Hit
CTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTG1152800.11322019517892734No Hit
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC1106620.10868470887309557No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1100440.10807775119942645No Hit
TCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGG1098320.10786953918192183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)