FastQCFastQC Report
Tue 14 Feb 2023
EGAF00001879420

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001879420
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101782906
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT3640140.35763765675937764No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG3019390.29665000918720086No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT2717350.26697508518768365No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT2248960.2209565523703951No Hit
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC2027530.19920142582684758No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA1881800.18488369746487684No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC1669230.16399905107838048No Hit
GCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACC1647790.16189260699630642No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA1596140.1568180810243323No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAC1524960.14982476527050623No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1474580.14487501467093108No Hit
CGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG1421780.13968750312552483No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG1256360.12343526524974636No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1230880.12093189793578896No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1132380.111254437950514TruSeq Adapter, Index 12 (100% over 50bp)
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG1126640.11069049256660053No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT1098480.10792381974238385No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1093020.10738738388939298No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC1052520.10340832673808703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)