FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001879720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001879720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23596785
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCCCTAATCTCGTA7292103.090293868423177Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC940430.3985415809823245Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA936890.3970413766112629Illumina Paired End PCR Primer 2 (100% over 32bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG236960.10042045982111546Illumina Paired End PCR Primer 2 (100% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)