FastQCFastQC Report
Tue 13 Sep 2022
EGAF00001879722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001879722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12090220
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGCCCTAATCTCGTA6156745.092330826072644Illumina Paired End PCR Primer 2 (97% over 39bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGA736920.6095174446784261Illumina Paired End PCR Primer 2 (100% over 32bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGC656630.5431083967041129Illumina Paired End PCR Primer 2 (97% over 36bp)
CAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCG188480.15589459910572348Illumina Paired End PCR Primer 2 (100% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGCA140280.11602766533611464Illumina Paired End Adapter 2 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)