FastQCFastQC Report
Wed 8 May 2024
EGAF00001879723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001879723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12090220
Total Bases616.6 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1870771.5473415702940063Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCATTCCTGCTGAACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT739810.6119078064749855Illumina Single End PCR Primer 1 (100% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG297030.24567791156819316Illumina Single End PCR Primer 1 (98% over 50bp)
CGGCATTCCTGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA194610.16096481288181688Illumina Single End PCR Primer 1 (100% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)