FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492715
Sequences flagged as poor quality0
Sequence length251
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1282438.591258210710015No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA1158317.7597532013813755No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT818865.485708926352318No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA545313.653142093433777No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA514433.4462707214706088No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT504313.3784747925759437No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA447572.998362044998543No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA368422.4681201702937265No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT331762.222527408112064No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA328692.2019608565600266No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA207041.3870028773074565No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT186871.2518799636903226No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA183291.2278968188837118No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT181201.2138954857424225No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT134810.9031194836254743No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA130970.8773945461792774No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT126640.8483869995277062No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA120620.8080578007188245No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT112780.7555360534328388No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA102840.6889459809809642No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT84820.5682263526527167No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA79860.5349983084513789No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA77230.5173794059817178No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT73290.49098454828952615No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA67680.4534020224892227No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA65560.43919971327413476No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT63050.42238471509966735No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA60950.4083163899337784No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT56680.37971079542980407No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA55290.37039890401047754No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT52490.35164113712262557No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT47120.3156664199127094No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT46660.31258478678113366No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA45780.30668948861638023No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT44310.2968416610002579No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA44190.29603775670506427No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT43670.29255417142589174No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT41300.276677061595817No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT39290.2632116646513233No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA37670.2523589566662089No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGT35340.23674981493453204No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA34890.23373517382755585No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT34230.22931370020399072No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA33650.22542816277722139No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA33420.22388734621143352No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA32440.21732212780068533No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT31440.21062292534073818No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT30430.20385673085619158No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT28700.19226711060048302No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT28540.19119523820689147No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGT27990.18751067685392053No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA27940.1871757167309232No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA26780.1794046418773845No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA26330.17639000077040828No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGT25670.17196852714684316No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC25490.17076267070405268No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA24830.16634119708048759No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA23410.15682832958736265No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT23070.1545506007509806No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT22770.15254084001299645No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGGGGT22600.15140197559480542No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA22560.15113400749640754No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTG20940.14028129951129317No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA20790.1392764191423011No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA18640.12487313385341474No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGT18570.12440418968121845No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA18020.12071962832824752No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAAAGACGGTGACCGTGGT17930.12011670010685228No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT17790.11917881176245967No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATGGT17750.1189108436640618No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTG17520.11737002709827395No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT17080.11442237801589722No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGAGACCAGGGT16760.11227863322871412No Hit
GNCAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA16510.11060383261372733No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCGGGGT16160.10825911175274584No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT16080.10772317555595007No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGACACGGTGACCAGGGT15680.10504349457197121No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA15470.10363666205538231No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCAGGGT15210.10189486941579605No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)