FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650258
Sequences flagged as poor quality0
Sequence length251
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA1109926.725736218215577No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT1082326.55848964222564No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT910845.519379394009906No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT639083.8726065863640717No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA603803.6588218327073707No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA440262.6678252733814958No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA348592.1123363740699936No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT342512.075493650083805No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA297531.8029302084886119No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT287321.7410610946894365No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT247071.4971598380374462No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA241731.4648012613785237No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT205191.2433813379483694No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA203751.2346554296358507No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCA168181.0191133749995454No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT152460.9238555425878863No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGA114930.6964365571928752No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT109960.6663200541975861No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA99500.6029360257608204No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGATGACCGTGGT98880.5991790374595972No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT96300.5835451183996685No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT94720.5739708578900996No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT94070.5700320798323656No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCA93970.5694261139773296No Hit
NACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT81100.49143830843419634No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCATGGT77380.4688963786268571No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT76780.4652605834966412No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA74620.4521717210278635No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT71130.4310235126871071No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGT68200.4132687131345523No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGA64450.39054499357070227No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGA63260.3833339998957739No Hit
GANAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT60410.36606397302724786No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT55800.33812894711008823No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGAT54850.33237227148724624No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCA54060.3275851412324618No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGT52550.3184350568214182No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT51770.31370852315213743No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGA51580.31255718802756904No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT50870.3082548304568134No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA50650.3069217055757342No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGT48480.293772246521453No Hit
GNCAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT43680.264685885479725No Hit
GANAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT41880.253778500089077No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGATGACCGTGGT40080.24287111469842898No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA38350.2323879054063062No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGATGACCAGGAT37940.22990344540065855No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGT37410.22669182636896773No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA35370.21433012292623332No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT33440.20263498192403853No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAA31780.19257594873044095No Hit
GACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATC31600.19148521019137613No Hit
GNCAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGT31460.19063685799432573No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGACCCTGCT30230.18318347797738294No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGT30040.18203214285281452No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT29590.17930529650515253No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAA29520.17888112040662732No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGA29430.17833575113709493No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA29370.17797217162407333No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC29060.17609367747346172No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA28220.17100356429115932No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGT26830.16258063890615893No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGT26730.16197467305112292No Hit
GACAAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGAGT26000.1575511223093601No Hit
NACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA25050.15179444668651812No Hit
NACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT24950.15118848083148212No Hit
GACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGCTCTACCCC24920.1510066910749713No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGATGACCGTGGT24850.15058251497644612No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGATGACCGTGGT23570.14282615203198532No Hit
GANAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT23060.1397357261713017No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGT23030.13955393641479089No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGAGT22570.1367664934816253No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGATGACCAGGAT22450.1360393344555821No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGGGGT22120.1340396471339633No Hit
GANAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGA22040.1335548744499345No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGT21890.13264592566738048No Hit
GNCAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGA21400.1296766929777041No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACAAGGGT21310.1291313237081717No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTA20880.1265256705315169No Hit
GACAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCATGGT20770.12585910809097728No Hit
NACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGT20570.12464717638090529No Hit
GANAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGA19990.12113257442169648No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGAGATTATTGT19220.11646663733791927No Hit
GANAAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGT19040.11537589879885449No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGCAGAGACGGTGACCATTGT18830.11410337050327887No Hit
NACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT17080.10349896804014887No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGT16850.10210524657356607No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTCACCAGGGT16830.10198405340255887No Hit
GACAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGA16800.10180226364604808No Hit
GACAAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGC16800.10180226364604808No Hit
NACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGA16790.10174166706054448No Hit
GNCAAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGT16600.10059033193597607No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)