FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276628
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC640605.017906547561232No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC420883.2968100339331423No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC303432.376808279310809No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC294442.306388391919964No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC256042.0055959919412705No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC234751.8388285389322494No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC233631.8300554272662042No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC228911.7930830281021566No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC196871.542109369369934No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC130531.022459165865076No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC124940.9786719388890107No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC123160.9647289578483317No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC111360.8722979599382122No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC111010.8695563625425731No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC109950.8612532390014945No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC77350.6058930244362493No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC59460.46575823184200876No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC58360.45714178288428575No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC56060.43912557142722863No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC52230.4091246627835204No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGCGACCAGGGTTCC50090.39236175299304105No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT50070.39220509028471884No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC49470.38750520903505176No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGCGACGGTGACCGTGGTCCC43550.3411330473716698No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC42020.32914835018501865No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC37610.2946042229999655No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTGCC35350.2769013369595528No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC33910.2656216219603518No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC29210.22880588550462624No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC27570.2159595434222029No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC27140.21259129519327477No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC26310.206089792797902No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGCGACCAGGGTTCC22700.1778121739457383No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAGGAGACGGTGACCGTGGTCCC21340.16715910977982623No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGAGCATTGTCCC21220.16621913352989282No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC21130.16551415134244274No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGCGACGGTGACCGTGGTCCC19950.1562710515514308No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGCGGAGACGGTGACCAGGGTGCC19860.15556606936398074No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC19410.1520411584267304No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC19320.15133617623928036No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC18410.14420802301061858No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGATGACCGTGGTCCC18260.14303305269820182No Hit
AAGACCGATGGGCCCTTGGTGGAAGGTGAGGAGACGGTGAGCAGGGTCCC18130.1420147450941073No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC18060.14146642561497946No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG17240.13504325457376776No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTGCC16590.1299517165532951No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC15910.12462518447033905No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTAAGGAGACGGTGACCGTGGTCCC15380.12047362269979978No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGTTCC14360.11248382457536572No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGAGGTCCC14040.10997722124220993No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC13920.10903724499227653No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTCGGGCTGCTGG13910.10895891363811541No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC13780.10794060603402088No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG13670.10707896113824858No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC13520.10590399082583181No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCAGGGTTCC13520.10590399082583181No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC12940.10136077228448694No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)