FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757453
Sequences flagged as poor quality0
Sequence length250
%GC55

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC889975.06397610633115No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC819804.664705115869386No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC522702.9741904904427034No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC521772.968898741531068No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC432082.4585579244508957No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC411112.3392375215724117No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC269051.5309086501886537No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC261021.4852175278656101No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC258711.4720735063754196No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC213611.2154521344240785No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC204071.1611690326853692No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC178431.0152760841968462No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGTGCC165160.9397690862856646No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGTTCC155160.8828685603541034No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC143490.8164656465919715No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC138450.7877877815224646No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC131450.7479574133703717No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC118660.675181640703905No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC117300.6674431691772127No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT116230.6613548129025357No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGTTCC112890.6423500372413942No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC99690.5672413430117335No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGTTCC95820.5452208394762192No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAATGAGGACGGAGGAGAC87470.4977089003233657No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGTGCC82780.4710225536614635No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGTTCC81150.461747767934619No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC70910.4034816293807004No Hit
AAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCGGGGTGCC67830.38595626739377953No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC63410.3608062349320295No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGTTCC61940.35244185762009No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC61290.3487433234345385No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC57680.3282022335732449No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGTTCC56830.3233656888690622No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGTTCC56780.3230811862394044No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC54180.3082870494971985No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC52320.29770355167392815No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGTTCC50710.28854256699894676No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC50350.28649414806541057No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGTTCC50000.2845026296578059No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCAATGTTCC49360.28086099599818604No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAAATGAGGACGGAGGAGAC45630.2596370998257137No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC44320.25218313092867917No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGTTCC42650.24268074309810847No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGC41110.23391806210464805No Hit
AAGACGGATGGGCCCTTGGTGGAAGCCGAGGAGACAGTGACCGGGGTGCC35340.20108645864213723No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGTCCC34070.19386009184882896No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGCGTTCC32790.18657682452958912No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC32780.18651992400365755No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCCAGGAGACGGTGACCGTCGTCCC31750.18065916983270675No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC30930.17599332670631876No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCGTGGTCCC30830.17542432144700312No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTAGTCCC30220.1719533893651779No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGTTCC29880.17001877148350483No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGATGAGACGGCGACCAGGGTTCC29520.16797035254996862No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC28580.16262170311240187No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC28510.16222339943088093No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC28040.15954907471209756No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCGTGGTCCC27940.15898006945278195No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGTGTCCC27830.15835416366753477No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAAGGTTCC27780.15806966103787698No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTACAACTGTGAGTC27670.1574437552526298No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGCGACTAGGGATCC27470.15630574473399855No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCTGGGTTCC27150.15448492790418863No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC26170.14890867636289562No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCAATGTTCC26030.14811206899985377No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGTTCC25380.1444135348143023No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC23820.13553705276897876No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGTTCC22580.12848138755346517No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC22140.12597776441247646No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCTTTGTCCC20630.11738578499681074No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC18900.10754199401065064No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGTCCC18270.10395726087696229No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGTGCC17750.1009984335285211No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)