FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1731037
Sequences flagged as poor quality0
Sequence length250
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC33233819.198780846394385No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC1422918.219986054601952No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC576663.3312979445268933No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC503032.905945973425178No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC271081.5659977227523154No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC260771.506438048406822No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGCGGTTCC185231.070052228808512No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC156190.9022915165880336No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC119610.6909730987841393No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC118300.6834053807053229No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC108380.6260986911313854No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC98780.5706406044469298No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGCGGTTCC83530.4825431229950602No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC81000.4679276064000943No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC80990.467869837559798No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC72680.4198639312735661No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGTTCC67180.3880910691105967No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACAGTGACCATTGTCCC63500.3668321358815554No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC62920.36348154314436953No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC62610.36169070909518397No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC55430.3202126817624349No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC54200.313107114405989No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT48040.2775215087834633No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC44500.25707133931857035No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC44260.25568488715145893No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC42100.2432068176474564No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC37220.21501562358285814No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC33520.1936411526732242No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGTCCC33390.19289015774937218No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGTGCC33020.19075271065840882No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGTTCC32120.18555351503174108No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC31790.18364714330196294No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACAGTGACCATTGTCCC29040.16776071222047825No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC28390.16400573760121823No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC26890.15534041155677203No Hit
AAGACGGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC26310.15198981881958618No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCGTGGTCCC25960.1499679094092154No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC25740.1486969949226966No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGC25410.14679062319291847No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC25110.14505755798402922No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC24910.14390218117810305No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTCCC24670.1425157290109917No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGTTCC24250.14008943771854676No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGTGCC23550.1360456188978052No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC23450.1354679304948421No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC23000.13286833268150827No Hit
AAGACGGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC22830.13188626239647103No Hit
AAGACCGATGGCCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC22510.13003765950698914No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC22400.12940220226372978No Hit
AAGACCGATGGACCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC20930.1209101827401725No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGCGGTTCC20570.11883050448950543No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGGAGGGCTTTGTTGGAGACCTT19250.11120501757039278No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGTTCC17680.10213530964387242No Hit
AAGACCGATGGGCCCTTGGTGGAGACCTTGCACTTGTACTCCTTGCCATT17500.1010954705185389No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC17420.10063331979616842No Hit
AAGACGGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC17370.1003444755946869No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)