FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018455
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC20686910.248878473882252No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1216536.027035529650153No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC1041745.161076169644605No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC622923.086122801845966No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC609873.0214693911927686No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC546022.7051383360045183No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC422622.093779648295355No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC407622.019465383176737No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC230361.141268940848322No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGTTCC180640.8949419234018099No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC176830.8760661000616808No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC150380.7450252792358513No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC135220.6699183286226347No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC115720.5733097839684312No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC105460.5224788266272966No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC96500.4780884389297755No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGTTCC90750.44960130396763864No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG84630.41928108379924245No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC83150.41194874297420553No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC76090.37697149552504267No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGTTCC69190.3427869335704784No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC65530.32465425288153565No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC65180.3229202533621012No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGTGCC62670.3104849996655858No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCATTGTCCC53490.2650046694129916No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGTTCC52160.2584154712391408No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC41950.2078322281150682No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC38370.19009589017342474No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACCTTGCACTTGTACTC37300.18479480592829664No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGTTCC35300.17488623724581426No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGACGGTGACCAGGGTTCC34640.17161640958059507No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC34190.16938698162703653No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC33810.16750435357736487No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCAATGTTCC32770.16235189786247403No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC32070.15888389882360518No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGTGCC31330.15521772841108672No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAAGAGACGGTGACCAGGGTTCC29470.14600275953637806No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC29310.1452100740417795No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAACTCC28280.14010716117030106No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCATTGTCCC27430.13589601948024604No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGTTCC26330.13044630670488072No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC25670.12717647903966153No Hit
AAGACCGATGGGCCCTTGGTGGAGGGTGTGGAGACGGTGACCGTGGTCCC25170.12469933686904092No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC24730.12251945175889481No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAATGAGGACGGAGGAGAC24380.12078545223946038No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGAGGACGCTGAC23610.11697065329670465No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGTGCC23440.11612842495869365No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC22050.10924196972436839No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC21910.10854836991659463No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC21580.10691345608398503No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGTTCC20600.10205825742956867No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGTACC20200.10007654369307217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)