FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936859
Sequences flagged as poor quality0
Sequence length250
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC21315111.004982809796687No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC1025445.294345122696076No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC954814.929682542714777No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC458332.36635707607007No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC450632.32660198806418No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC408712.1101690933619843No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC407992.1064517344835116No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC232911.2025139672015361No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC217811.1245526907224532No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC196441.0142194140100027No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC190830.9852549927485688No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC161610.8343921782638798No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC144420.7456402350403412No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC141780.7320099191526074No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAAGAGACGGTGACCATTGTCCC124810.6443938355863799No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCTGGGTTCC108770.561579340571513No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC104990.5420632064595307No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC91940.47468607678721064No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC88810.4585258916627385No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGTGCC87270.4505748740615605No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGTTCC84280.43513750871901363No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGTTCC78430.4049339678314219No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC76570.3953307907287005No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC71960.3715293679095897No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC69920.36099685108725005No Hit
AAGACGGATGGGCCCTTGGTGGAGGCGGAAGAGACGGTGACCATTGTCCC62960.32506238192867937No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC56470.2915545220380007No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGTGCC52810.2726579477390972No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGTTCC49030.2531418136271148No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC45590.23538109898552242No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC44710.23083766035627787No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC44700.23078603037185466No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT43180.2229382727395231No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCAGGGTGCC42300.21839483411027855No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC42080.2172589744529674No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC38970.2012020492973417No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGACGAGACGGTGACCAGGGTTCC38930.20099552935964876No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAAACGGTGACCAGGGTTCC38730.1999629296711841No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCGTGGTCCC35070.18106635537228058No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC33960.17533542710130165No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGGGGAGACGGTGACCACAACTCC32930.17001753870570857No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAAACGGTGACCAGGGTTCC32830.16950123886147625No Hit
AAGACCGATGGGCCCTTGGTGGAGGCGGAAGAGACGATGACCAATGTTCC31430.1622730410422235No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGCGGTCCC31430.1622730410422235No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG30980.159949691743178No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC29100.15024325467161007No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC28570.14750686549717867No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTCCC28510.1471970855906393No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC26600.13733575856580163No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTCCC26390.13625152889291373No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC25240.13031408068424186No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTTCC25020.12917822102693072No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACAGTGACCGGGGTGCC24870.1284037712605822No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGATCC24040.12411848255345381No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGTTCC23850.12313751284941238No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC23510.12138209337902242No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC23020.11885222414228398No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGACGACCGTGGTCCC22630.11683865474977786No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC20190.10424093855050884No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC19580.10109150950069158No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGGGGTTCC19450.10042031970318956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)