FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001880101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248592
Sequences flagged as poor quality0
Sequence length250
%GC58

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC15003612.016415290182863No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC523034.188958442789959No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA423253.3898182913233468No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC333752.6730108794546177No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC321412.5741795558517113No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC297102.3794802465497136No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA294122.3556133628919613No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC260532.0865903353537423No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA228541.8303817419941821No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT221411.7732774196855337No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTCCC215381.7249830208747132No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA153241.2273024334610505No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC146901.1765252380281148No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC138691.1107711726488718No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC119130.9541147148147673No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTCCC110860.8878801081538246No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC110600.8857977625997925No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCCTTGTCCC87560.7012699104271051No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGC69480.5564668042082602No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC63530.5088131271063726No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC63470.5083325858246729No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC57350.4593173750913029No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC53650.42968399605315427No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC45120.36136704383817936No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCCTTGTCCC43520.3485526096595205No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC37810.3028210976844318No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACGGTTCC33720.2700642003152351No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC31870.25524751079616076No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC31460.2519638120378795No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCACCA30250.2422728961902687No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC28210.2259344926124787No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC26110.20911554775298896No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTCCC21680.1736355831208273No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGATGAGACGGTGACCAGGGTTCC21360.17107269628509553No Hit
AGCGACCTCGCGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC20260.16226277278726758No Hit
AGCGACCTCAGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC19910.15945961531068598No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTACCC19010.15225149608519037No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC18820.15072978202647463No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG18790.15048951138562475No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC16970.13591309250740033No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTCCCGTAC16490.13206876225380268No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC15190.12165703448364237No Hit
AAGACCGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTTCCT14950.11973486935684355No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTGCTCGAGCACTGTCAGCC14480.11597062931686251No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC13570.1086824198777503No Hit
AGCGACCTCGAGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC13210.10579917218755205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)