FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953322
Sequences flagged as poor quality0
Sequence length250
%GC57

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1600218.192248897007252No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC841684.308966980354493No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC728223.7281103678758547No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA720013.6860794072866634No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC469452.4033415893539316No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC434232.2230333759615672No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC403932.0679130220209467No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC384551.968697429302491No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC378391.9371614101515264No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGCTGTCCTTGGTCCTGGCTGAGA377761.9339361354656324No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC355331.8191061176805463No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC265561.3595300723587815No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC257051.3159632666810694No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC214381.0975149002571005No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC207921.0644430360176151No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC203061.0395623455835752No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA195170.9991696197554729No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGTGACCAGGGTTCC189970.9725483048877758No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGTACC183560.9397324148297106No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCGGAGACGGTGACCAGGGTTCC139900.7162157596136224No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC113500.581061391823775No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCTGTCCTTGGTCCTGGCTGAGA106300.5442011096992713No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC105340.539286405416004No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGACGAGACGGCGACCAGGGTACC99530.509542205534981No Hit
AAGACCGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTTCCT94460.4835864235389762No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC88080.45092411798976306No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGAGCAGGGTTCC58890.3014863908766706No Hit
AAGACGGATGGGCCCTTGGTGGAAGCGAGGTGGGGGTCTCAGCCCTTCCT52180.26713465573008444No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGCGACCAGGGTTCC47550.24343144653057713No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC47550.24343144653057713No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGACTC46870.2399501976632629No Hit
AAGACCGATGGGCCCTTGGTGGAAGTTCCTGAAGTTCCCTTTTCTTCTGG35760.18307273455170217No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT35620.18235600684372572No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCGTGGTCCC34570.17698054903390223No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC33920.1736528846754401No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGTGGCTGCTCGGTCCTGGTACCC33200.16996685646298973No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGAGACCCGGGCTCC31540.16146851363984024No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC28480.14580289373692612No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGCACCACAGGCTCTGGCTGACTC25530.13070041703313637No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC24060.1231747760993835No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC23140.11846485116125247No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC22130.11329417269656512No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGC19700.10085382747954509No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)