FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635150
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC24313014.86897226554139No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC1023226.25765220316179No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC757464.632357887655567No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC553553.385316331835No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC542103.3152921750298137No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC533443.262330673026939No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC339452.0759563342812584No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC288091.7618567103935416No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC274541.678989695135003No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC254551.5567379139528483No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC210591.2878940769959943No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC190781.1667431122526986No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATTACCCGGATTCC116420.7119836100663548No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC96740.5916276794177904No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC88910.5437421643274317No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC61350.37519493624438127No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT50190.3069443170351344No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATTACCCGGATTCC49990.3057211876586246No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC47970.2933675809558756No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC47780.2922056080481913No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC46890.2867626823227227No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC40890.2500688010274287No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC36380.2224872335871327No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC35460.21686083845518758No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGATACGGTGACCGTTGTCCC30420.18603797816714063No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC27880.17050423508546617No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG27510.16824144573892302No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC27260.16671253401828579No Hit
AAGACCGATGGGCCCTTGGTGGAGGTTGAGGAGACGGTGACCAGGGTTCC27070.16555056111060149No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC26650.1629819894199309No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC26040.159251444821576No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC25400.15533743081674464No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGGCGGTGACCGTGGTCCC25330.15490933553496622No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC24760.15142341681191326No Hit
AAGACCGATCGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC23850.14585817814879368No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC22890.13998715714154664No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGC20790.12714429868819374No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACTAGGGTTCC20610.12604348224933493No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC20080.12280218940158395No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG20070.12274103293275847No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGCGGCGT19670.12029477417973887No Hit
AAGAGCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC18600.11375103201541144No Hit
AAGACGGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC17180.10506681344219185No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)