FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481177
Sequences flagged as poor quality0
Sequence length250
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC16383111.060865784440347No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC688324.647115098330584No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC621384.195177213796866No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC606714.096134358013931No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC557183.761738131229421No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC406572.7449116479664486No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC369792.4965956128133233No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC366972.477556699840735No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC249521.6846062286951526No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC242221.6353210993689478No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC241231.628637225665805No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC239361.6160121308932018No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC181021.2221361795382997No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC177321.1971560454962507No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC158081.0672593484775958No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGTCCC121220.8184032023181566No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC105740.7138917225962866No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC73400.4955518482936206No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC67700.4570689390937072No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC66480.44883224624741No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGATGACCATTGTCCC54660.36903084506443184No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC54400.3672754842939095No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT51560.34810154356974216No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATTACCCGGATTCC42150.2845709864519905No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGCGTTCC33070.22326838723528653No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC26000.17553607705223617No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC24910.1681770645912001No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC22630.15278390091123478No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTGCC22570.15237881765649886No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCGTGGTCCC21680.14637008271124924No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC20530.13860598699547724No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATTACCCGGATTCC18200.12287525393656532No Hit
AGCGACCTCGGGTGGGAACACCTTTTTCAGGTCCTCTAGCACGGTGAGCC16940.11436850558711079No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC16890.11403093620816418No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC16740.11301822807132436No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGCTGACCATTGTCCC16720.11288320031974572No Hit
AAGACCGATGGGCCCATGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC16330.11025015916396218No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTACCCCACTGTGCA15880.1072120347534427No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACGAGGGTTCC15110.10201346631766495No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACCGTACT15040.1015408691871397No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGCGTTCC14950.10093324430503578No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)