FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001880107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001880107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1914303
Sequences flagged as poor quality0
Sequence length250
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC19788610.337235014519646No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC838624.380811188197479No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACTGTCAGCC791304.133619390451773No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTAGCACGGTGAGCC710653.712317224598196No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC703053.6726160905561973No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGTGACCGTGAGCC687513.5914377191071636No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTAGGATGGAGAGTC359111.8759308218186985No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACTGTGAGTC352611.8419759045459365No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACGGTTAACC326151.7037532720786626No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGTCCC308791.6130675237932552No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCGAGCACCAGGAGCC266531.392308323186037No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGTACGGTCAGCC251381.3131672467733688No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC230331.2032055531438859No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACTGAGAGCC128720.672411838669218No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC103920.5428607696900648No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCATTGTCCC102560.5357563562299176No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC93530.48858514038791145No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCCAGCACGGTCAGCC83980.4386975311640843No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC81600.42626480760882685No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCCAAGACAGAGAGCT75510.39445166204096216No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGTCACAGTGAGCC66030.3449297211569955No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCCAGGCACTGTCC65220.3406984160814667No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTCCAGGCACTGTCC61650.32204933074858055No Hit
AAGACCGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC56520.29525106527023154No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC48660.25419173453732247No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC45800.2392515709373072No Hit
AAGACCGATGGGCCCTTGGTGGAAGCCGAGGAGACGGTGACCGTGGTCCC45620.23831128092052303No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCCGGGTTCC44190.23084119912051543No Hit
AAGACCGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC44120.23047553078065489No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGATGGTGACCAGGGTTCC43680.22817704407296024No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGCTCC37170.1941698884659325No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGAAAGGGAAGAG35910.18758785834844327No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGATTCC35790.18696099833725383No Hit
AAGACCGATGCGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC35020.18293864659878817No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTACAACAGTGAGCC34900.18231178658759872No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTCCC31090.16240898123233363No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGCTGACCAGGGTTCC28960.15128221603372088No Hit
AAGACCGATAGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC28320.1479389626407105No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTGGAAAGGGAAGAG28200.14731210262952105No Hit
AAGACGGATGGGCCCTTGGTGGAAGCTGAGGAGACGGTGACCAGGGTTCC25230.13179731735258213No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACAATTGTCCC25040.13080478900153214No Hit
AGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTCTGGTCAATATCTGA24150.12615557725187704No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAAGAGACGGTGACCAGGGTTCC24000.12537200223789025No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTGCC23520.12286456219313244No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGCTTCC22860.11941683213159045No Hit
AGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTCTCTGCCCATCCCGC22730.11873773378613521No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCATTGTCCC22310.11654372374697212No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCACCGTACT22240.11617805540711162No Hit
AAGACCGATGAGCCCTTGGTGGAGGCTGAGGAGACGGTGACCAGGGTTCC21580.11273032534556963No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGATGAGATGGTGACCGTGGTCCC21400.11179003532878547No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGAGGAGACGGTGACCGTGGCCCC21100.11022288530081184No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACAGTGACCAGGGTGCC20210.10557367355115675No Hit
AAGACCGATGGGCCCTTGGTGGAGGCTGGAGAGACGGTGACCATTGTCCC20060.10479009853716992No Hit
AAGACGGATGGGCCCTTGGTGGAGGCCGAGGAGACGGTGACCAGGGTTCC19950.10421547686024626No Hit
AAGACGGATGGGCCCTTGGTGGAAGCCGAGGAGACGGTGACCGTGGTCCC19840.1036408551833226No Hit
AAGACGGATGGGCCCTTGGTGGAGGCTGAGGAGACGATGACCAGGGTTCC19580.10228265849241212No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)